维生素D受体在肺腺癌高表达及其对肺腺癌细胞活力和转移能力影响的研究  

High expression of vitamin D receptor in lung adenocarcinoma and its influence on viability and metastasis ability of lung adenocarcinoma cells

作　　者：邹淑梅 包振明 叶嘉 余宗阳[2,3] 宋颖芳 ZOU Shumei;BAO Zhenming;YE Jia;YU Zongyang;SONG Yingfang(Dongfang Hospital of Xiamen University,School of Medcine,Xiamen University,Xiamen 361000,China;Department of Pulmonary and Critical Care Medicine,Fuzong Clinical College of Fujian Medical University,Fuzhou 350025,China;Department of Respiratory,The 900 Hospital of the Joint Logistics Team,Fuzhou 350025,China)

机构地区：[1]厦门大学附属东方医院,福建厦门361000 [2]福建医科大学福总临床医学院,福建福州350025 [3]联勤保障部队第900医院呼吸内科,福建福州350025

出　　处：《中国病理生理杂志》2024年第8期1384-1391,共8页Chinese Journal of Pathophysiology

基　　金：福建省自然科学基金面上项目(No.2020J011137);联勤保障部队第九〇〇医院临床医学研究专项(No.2020L13)。

摘　　要：目的:探究维生素D受体(vitamin D receptor,VDR)在肺腺癌中的表达及其对肺腺癌细胞生物学功能的影响。方法:本研究通过6个肺腺癌数据集(共792例肺腺癌组织和230例相邻非肿瘤组织)分析了VDR的mRNA在肺腺癌中的表达水平及其与预后的关系,免疫组化检测30例肺腺癌患者肿瘤组织和癌旁组织中VDR蛋白表达。以肺腺癌细胞A549和H1650为研究对象,分别转染阴性对照和两条VDR短发夹RNA(shRNA)。CCK-8实验比较各实验分组的细胞活力,Transwell实验和划痕实验比较各实验分组细胞的侵袭及迁移能力。基因集富分析肺腺癌VDR高表达的组织富集的相关信号通路。结果:VDR的mRNA在肺腺癌组织中的表达显著增加(P<0.01)。免疫组化实验进一步证实了VDR在肺腺癌中显著高表达(P<0.01)。生存分析结果显示VDR的表达对肺腺癌患者的整体生存率没有显著影响(P>0.05)。敲减VDR可以显著抑制肺腺癌的细胞活力、侵袭及迁移能力(P<0.05)。基因集富集分析结果显示VDR高表达的肺腺癌组织显著富集在上皮-间充质转化等信号通路(P<0.05)。结论:VDR在肺腺癌中高表达,敲减VDR可以抑制肺腺癌细胞活力、侵袭及迁移能力。AIM:To explore the expression level of vitamin D receptor(VDR)in lung adenocarcinoma and its impact on the biological function of lung adenocarcinoma cells.METHODS:The mRNA expression level of VDR in lung adenocarcinoma and its relationship with prognosis were analyzed through 6 lung adenocarcinoma datasets(comprising a total of 792 lung adenocarcinoma tissues and 230 adjacent non-tumor tissues).Immunohistochemistry was used to detect VDR protein expression in 30 lung cancer patients.Lung adenocarcinoma cell lines A549 and H1650 were studied,with transfection of negative control(NC)or two VDR short hairpin RNAs(VDR-shRNAs).CCK-8 assay compared the cell viability of cells in each experimental group.Transwell and wound-healing assays compared the invasion and migration capabilities.Gene set enrichment analysis identified pathways enriched in lung adenocarcinoma tissues with high VDR expression.RESULTS:The mRNA expression level of VDR was significantly increased in lung adenocarcinoma tissues(P<0.01).Immunohistochemistry further confirmed the high expression of VDR in lung adenocarcinoma(P<0.01).The survival analysis showed that the expression of VDR had no significant effect on the overall survival of lung adenocarcinoma patients(P>0.05).Knockdown of VDR significantly inhibited the cell viability,invasion,and migration capacity of lung adenocarcinoma cells(P<0.05).Gene set enrichment analysis showed that lung adenocarcinoma tissues with high VDR expression were enriched in signaling pathways such as epithelial-mesenchymal transition(P<0.05).CONCLUSION:VDR is highly expressed in lung adenocarcinoma,and VDR knockdown can inhibit the cell viability,invasion,and migration capacity of lung adenocarcinoma cells.

关 键 词：维生素D受体 肺腺癌 细胞活力 细胞侵袭 细胞迁移 

分 类 号：R734.2[医药卫生—肿瘤] Q565[医药卫生—临床医学] R363.2[生物学—生物化学]