基于网络药理学探讨三化汤改善便秘的作用机制  

Exploring the Mechanism of Sanhua Decoction to Treat Constipation by Network Pharmacology

作　　者：田小昌 龚雨舒 丁祥武 TIAN Xiaochang;GONG Yushu;DING Xiangwu(School of Medicine,Jianghan University,Wuhan 430056,Hubei,China;Department of Gastroenterology,Wuhan Fourth Hospital,Wuhan 430033,Hubei,China)

机构地区：[1]江汉大学医学部,湖北北武汉430056 [2]武汉市第四医院消化内科,湖北武汉430033

出　　处：《江汉大学学报（自然科学版）》2024年第4期62-70,共9页Journal of Jianghan University：Natural Science Edition

摘　　要：目的 基于网络药理学方法研究三化汤治疗便秘的潜在作用机制。方法 利用TCMSP筛选出三化汤的活性成分,通过Cytoscape 3.9.1软件构建药物“药物-成分”网络;利用Genecards检索便秘的相关靶点,利用String平台进行蛋白质相互作用分析,构建PPI网络并挖掘网络中潜在的蛋白质功能模块。通过Metascape平台分析“药物-成分-靶点”及其参与的生物过程和通路。结果 三化汤的主要活性成分为柚皮素、木犀草素、芦荟大黄素、欧前胡素、新橙皮苷等;核心靶点有ATK1、TP53、IL-6、TNF、CASP3等;生物学通路主要作用于PI3K-AKT信号通路、AMPK信号通路以及NF-κB通路等。结论 初步揭示了三化汤治疗便秘的多成分、多靶点、多通路的作用机制,可为三化汤的临床利用开发提供基础。Objective To explore the potential mechanism of"Sanhua decoction"treating constipation using network pharmacology.Methods The active ingredients of Sanhua decoction were screened out by TCMSP,and the drug"drug-ingredient"network was constructed through Cytoscape 3.9.1 software.Genecards were used to retrieve relevant targets of constipation,and the String platform was used to analyze protein interactions,build PPI networks,and explore potential protein functional modules in the network.The"drug-componenttarget",the biological processes,and pathways involved were analyzed through the Metascape platform.Results The results showed that the main active ingredients of Sanhua decoction were naringenin,luteolin,aloe emodin,ammidin,and neohesperidin.The core targets were ATK1,TP53,IL-6,TNF,CASP3,etc.The biological pathway mainly acted on the PI3K-AKT signaling pathway,the AMPK signaling pathway,and the NF-kB pathway.Conclusion This study preliminarily reveals the multi-component,multi-target,and multi-pathway mechanism of Sanhua decoction in treating constipation,which provides a basis for the clinical utilization and development of Sanhua decoction.

关 键 词：三化汤 便秘 网络药理学 作用机制 

分 类 号：R285[医药卫生—中药学]