Decoding exercise-induced atomic components and prognostic shifts in endometrial carcinoma through differentially expressed genes  

作　　者：Yan Li Zhuo Wang Hai-Ning Li Yuan-Yuan Yong Min Kong Ngenzi Richard Djurist Xin Yang Sana Mushtaq Yan Ma Zhi-Fang Wang Ayang Mba Teresa Yue-Yue Shi Yao He Suo-Fei Li 

机构地区：[1]Department of Gynechology,General Hospital of Ningxia Medical University,Yinchuan 750000,China [2]Department of Gynechology and Obstetrics,Affiliated Beijing Chaoyang Hospital of Capital Medical University,Beijing 100020,China [3]First Clinical Medical College,Ningxia Medical University,Yinchuan 750000,China.

出　　处：《Traditional Medicine Research》2024年第11期51-64,共14页TMR传统医学研究

基　　金：supported by the Science and Technology Beneficiary Program of Ningxia Hui Autonomous Region(No.2023CMG03027);the Ningxia Key Research and Development Program(No.2022BEG03167);the National Natural Science Foundation of China(No.82060275).

摘　　要：Background:This study aimed to portray the atomic intelligence and prognostic implications of differentially expressed genes and their involvement in biological pathways in endometrial carcinoma,with a specific focus on the impacts of exercise on cancer.Methods:We utilized a multi-faceted approach,including volcano plots,Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses,Venn diagrams,protein-protein interaction networks,Kaplan-Meier survival analysis,Gene Set Variety Analysis,and single-cell transcriptomic analysis.Furthermore,we profiled tumor mutational scenes,assessed the prognostic value of immune-related features,and conducted a comprehensive examination of genetic variations and their impact on tumor mutational burden across different cancer types.Multidimensional genomic interactions and methylation elements were also investigated.Using real-time quantitative PCR and immunofluorescence staining,the effects of B-cell lymphoma 2(BCL2)silencing on TNF-αand caspase-3 gene expression were evaluated.Results:Our study identified a noteworthy number of differentially expressed genes in endometrial carcinoma with potential links to athletic performance traits.BCL2 expression levels were found to be associated with survival outcomes,and its changeability across cancers was related to immune cell infiltration and immune checkpoint gene expression.Single-cell investigations uncovered cellular complexity within tumor microenvironments and critical biological pathways in BCL2-overexpressing cells.The expression flow and mutational effect of BCL2 in endometrial carcinoma were characterized,and the prognostic implications of immune-related features were assessed.Hereditary variations,including copy number variations and their relationship with gene expression and tumor mutational burden,were investigated.Multidimensional genomic transaction highlighted the essential role of regulatory genes in cancer pathogenesis.Silencing of the BCL2 gene significantly inhibited the proliferation of HEC-108 c

关 键 词：endometrial carcinoma differentially expressed genes pathway enrichment immune pathways prognostic implications exercise and cancer 

分 类 号：R737.33[医药卫生—肿瘤]