Single-cell transcriptomes provide insights into expansion of glial cells in Bombyx mori  

作　　者：Xiaojing Liu Zhongjie Zhang Bo Hu Kai Chen Ye Yu Hui Xiang Anjiang Tan 

机构地区：[1]Jiangsu Key Laboratory of Sericultural Biology and Biotechnology,School of Biotechnology,Jiangsu University of Science and Technology,Zhenjiang,Jiangsu Province,China [2]Key Laboratory of Silkworm and Mulberry Genetic Improvement,Ministry of Agriculture and Rural Affairs,The Sericultural Research Institute,Chinese Academy of Agricultural Sciences,Zhenjiang,Jiangsu Province,China [3]Guangzhou Key Laboratory of Insect Development Regulation and Application Research,Institute of Insect Science and Technology and School of Life Sciences,South China Normal University,Guangzhou,China

出　　处：《Insect Science》2024年第4期1041-1054,共14页昆虫科学（英文版）

基　　金：supported by the National Science Foundation of China(32000339,32000336 and 31925007);the Natural Science Foundation of Jiangsu Province(BK20231249,BK20221292 and BK20210880)。

摘　　要：The diversity of cell types in the brain and how these change during different developmental stages,remains largely unknown.The life cycle of insects is short and goes through 4 distinct stages including embryonic,larval,pupal,and adult stages.During postembryonic life,the larval brain transforms into a mature adult version after metamorphosis.The silkworm,Bombyx mori,is a lepidopteran model insect.Here,we characterized the brain cell repertoire of larval and adult B.mori by obtaining 50708 single-cell transcriptomes.Seventeen and 12 cell clusters from larval and adult brains were assigned based on marker genes,respectively.Identified cell types include Kenyon cells,optic lobe cells,monoaminergic neurons,surface glia,and astrocyte glia.We further assessed the cell type compositions of larval and adult brains.We found that the transition from larva to adult resulted in great expansion of glial cells.The glial cell accounted for 49.8%of adult midbrain cells.Compared to flies and ants,the mushroom body kenyon cell is insufficient in B.mori,which accounts for 5.4%and 3.6%in larval and adult brains,respectively.Analysis of neuropeptide expression showed that the abundance and specificity of expression varied among individual neuropeptides.Intriguingly,we found that ion transport peptide was specifically expressed in glial cells of larval and adult brains.The cell atlas dataset provides an important resource to explore cell diversity,neural circuits and genetic profiles.

关 键 词：brain NEUROPEPTIDE SILKWORM single-cell transcriptome 

分 类 号：Q96[生物学—昆虫学]