过表达miRNA-10a促进肝硬化大鼠肝切除后的肝再生  

The overexpression of miRNA-10a promotes liver regeneration in rats with liver cirrhosis after hepatectomy

作　　者：余贞岑 顾小晖 刘超[1] 王鑫[2] Yu Zhencen;Gu Xiaohui;Liu Chao;Wang Xin(General Surgery,Second Affiliated Hospital of Zhengzhou University,Zhengzhou 450014,Henan,China;Hepatobiliary and Pancreatic Surgery,Fifth Clinical Medical College of Zhengzhou University,Zhengzhou 450052,Henan,China)

机构地区：[1]郑州大学第二附属医院普外科,郑州450014 [2]郑州大学第五附属医院肝胆胰外科,郑州450052

出　　处：《肝癌电子杂志》2024年第2期48-52,共5页Electronic Journal of Liver Tumor

基　　金：河南省医学科技攻关计划联合共建项目(LHGJ20220457)。

摘　　要：目的:探讨miRNA-10a对肝硬化大鼠肝切除后肝再生的影响。方法:将36只肝硬化大鼠随机分为假手术组(A组)、70%肝切除未转染组(B组)、70%肝切除转染组(C组),每组12只。术前2周成功将miRNA-10a肝向性转染C组大鼠;于术后12、24、48、72 h每组各处死3只大鼠,取腹主动脉血检测各组大鼠血清谷丙转氨酶(ALT)、白蛋白(ALB)的变化,PCR法检测肝组织中miRNA-10a的水平,计算不同时间大鼠的肝体积比。结果:术后12、24、48、72 h时C组大鼠肝组织中miRNA-10a水平显著高于其他两组,表明已经成功过表达了miRNA-10a;术后12、24、48、72 h时C组大鼠的血清ALT水平均低于B组,ALB水平均高于B组,表明miRNA-10a有利于保护肝功能;术后48、72 h时,C组大鼠的肝体积比显著高于B组,表明过表达miRNA-10a有利于肝组织生长。结论:过表达miRNA-10a能促进肝硬化大鼠肝切除后的肝再生。Objective:To investigate the effect of miRNA-10a on liver regeneration in rats after liver resection in liver cirrhosis.Methods:Thirty-six cirrhotic rats were randomly divided into a sham operation group(Group A,n=12),a 70%liver resection without transfection group(Group B,n=12),and a 70%liver resection with transfection group(Group C,n=12).Two weeks before surgery,successful hepatocyte-specific transfection of miRNA-10a was performed in rats of Group C.At 12h,24h,48h,and 72h after surgery,3 rats from each group were euthanized,and blood samples from the portal vein were collected for testing serum liver function indicators including alanine aminotransferase(ALT)and albumin(ALB).The levels of miRNA-10a in liver tissue were detected using PCR,and the liver-to-body weight ratio was calculated at different time points.Results:At 12 h,24 h,48 h,and 72 h after surgery,the levels of miRNA-10a in liver tissue of Group C rats were significantly higher than the other two groups,indicating successful overexpression of miRNA-10a.At 12 h,24 h,48 h,and 72 h after surgery,the serum ALT levels of Group C rats were lower on average than Group B,while the ALB levels were higher on average than Group B,suggesting that miRNA-10a is beneficial for protecting liver function.At 48 h and 72 h after surgery,the liver-to-body weight ratio of Group C rats was significantly higher than Group B,indicating that overexpression of miRNA-10a promotes liver tissue growth.Conclusion:Overexpression of miRNA-10a promotes liver regeneration in rats with liver cirrhosis after hepatectomy.

关 键 词：肝细胞癌 肝再生 miRNA-10a 大鼠 

分 类 号：R575.2[医药卫生—消化系统]