一株溶藻细菌Y3-5的溶藻特性及其应用研究  

Studies on the algicidal properties of an algicidal bacterium Y3-5 and its application

作　　者：戴楚涵 吴聪葱 李艳洁 李丽娜 赵雪婕 李欲如 韦彦斐 DAI Chu-han;WU Cong-cong;LI Yan-jie;LI Li-na;ZHAO Xue-jie;LI Yu-ru;WEI Yan-fei(Zhejiang Environmental Technology Co.,Ltd.,Hangzhou 310030,China)

机构地区：[1]浙江省环境科技有限公司,浙江杭州310030

出　　处：《环境生态学》2024年第8期83-92,98,共11页Environmental Ecology

基　　金：浙江省“尖兵”“领雁”研发攻关计划项目(2023C03132)资助。

摘　　要：对蓝藻水华的防控一直是一个备受关注的环境问题。本研究从嘉兴某养殖塘尾水分离获得一株铜绿微囊藻溶藻菌Y3-5,鉴定为侧孢短芽孢杆菌(Brevibacillus laterosporus)。研究了Y3-5对铜绿微囊藻的溶藻特性及其对实际藻华水体的除藻效果。研究表明,Y3-5的溶藻模式主要为通过分泌胞外活性物质的间接模式;对处于中浓度叶绿素a藻液(0.25 mg/L)的响应速度最快,溶藻率可达96.1%±0.88%;随着初始菌体密度的增加,Y3-5的溶藻响应速度提升,但对最终溶藻率影响不大;Y3-5的最适pH范围为7~8,且溶藻活性受pH影响较小;Y3-5在各个生长时期均有较好的溶藻活性,在对数后期和稳定期时溶藻效率有所提升;Y3-5在光照强度为4000 lux条件下的溶藻率高于2000 lux和黑暗条件,可达99.8%±0.08%。将Y3-5投加至实际藻类爆发水体11 d后,溶藻率达到86.4%,对水体中微囊藻、颤藻、浮丝藻等优势藻种的抑制率分别达到48.00%、91.07%、78.41%。综上,本研究分离的溶藻菌具有较高的溶藻活性以及实际应用价值,在蓝藻水华的实际治理方面具有一定潜力。The prevention and control of blue-green algae blooms has always been a highly concerned environmental issue.In this study,a Microcystis aeruginosa algicidal bacterium Y3-5 was isolated from the tail water of an aquaculture pond in Jiaxing,Zhejiang,and was identified as Brevibacillus laterosporus.The algicidal properties of Y3-5 against Microcystis aeruginosa and its algicidal removal effects on algal bloom water bodies were studied.Results showed that the algicidal mode of Y3-5 was mainly by secreting extracellular active substances.Y3-5 presented best algicidal response speed to the algal culture with medium concentration of chlorophyll a(0.25 mg/L),with an algicidal rate of 96.1%±0.88%.The initial cell density increased the algicidal response speed of Y3-5,but had little effect on the final algicidal rate.The optimum pH range of Y3-5 is 7~8 and the algicidal activity is less affected by pH.Y3-5 presented well algicidal performances in each growth period,and had better efficiency in the logarithmic later period and the stable period.The algicidal rate of Y3-5 under the light intensity of 4000 lux was higher than that under 2000 lux and dark conditions,reaching 99.8%±0.08%.After adding Y3-5 to the eutrophic water,the algicidal rate reached 86.4%,and the inhibition rate of the dominant algae species,such as Microcystis,Characteria and Fusarium,reached 48.00%,91.07%and 78.41%,respectively.In summary,the algicidal bacterium isolated in this study had high algicidal activity and practical application value,having certain potential in the actual treatment of blue-green algae blooms.

关 键 词：溶藻菌 溶藻特性 铜绿微囊藻 水华 

分 类 号：X172[环境科学与工程—环境科学]