羧甲基壳聚糖/燕麦β葡聚糖复合凝胶的制备及其对大鼠干槽症的治疗效果评价  

Preparation and efficacy evaluation of carboxymethyl chitosan/β-dextran composite gel for dry socket in rats treatment

作　　者：周霖 王慧 徐靖博 邹子川 孟箭 ZHOU Lin;WANG Hui;XU Jing-bo;ZHOU Zi-chuan;MENG Jing(Xuzhou Clinical College of Xuzhou Medical University,Xuzhou 221009;School of Stomatology,Xuzhou Medical University,Xuzhou 221004;School of Pharmacy,Xuzhou Medical University,Xuzhou 221004;Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy,Xuzhou 221004;Xuzhou Central Hospital.Xuzhou 221009,Jiangsu Province,China)

机构地区：[1]徐州医科大学徐州临床学院,江苏徐州221009 [2]徐州医科大学口腔医学院,江苏徐州221004 [3]徐州医科大学药学院,江苏徐州221004 [4]江苏省新药研究与临床药学重点实验室,江苏徐州221004 [5]徐州市中心医院,江苏徐州221009

出　　处：《上海口腔医学》2024年第4期354-359,共6页Shanghai Journal of Stomatology

基　　金：国家口腔疾病临床医学研究中心开放课题(NCRCO-202101)。

摘　　要：目的:探讨羧甲基壳聚糖(carboxymethyl chitosan,CMCS)/燕麦β葡聚糖(β-dextran,β-Dex)新型复合凝胶用于大鼠干槽症模型的治疗效果。方法:制备CMCS/β-Dex新型复合凝胶,对其黏度、外观形状及口腔湿润环境的稳定性进行评价。选取金黄色葡萄球菌进行体外抑菌实验,建立大鼠干槽症模型,分别设立空白阴性对照组、CMCS/β-Dex新型复合凝胶组及碘仿纱条阳性对照组,初步评价牙槽窝创口愈合效果。另取上颌骨及牙龈组织固定脱钙,组织病理学观察愈合情况,免疫荧光检测各组炎症因子的表达差异。采用SPSS 19.0软件包对数据进行统计学分析。结果:复合凝胶组有明显的抑菌作用,可有效发挥局部抗炎抗菌功效。与空白对照组相比,肉眼观察、免疫荧光及组织病理学观察均显示CMCS/β-Dex新型复合凝胶组具有显著的炎症抑制和促进创口愈合功能,与碘仿纱条组效果相当。结论:CMCS/β-Dex新型复合凝胶具有显著的抗菌和抗炎效果,能够加速大鼠干槽症创口愈合,有望为临床干槽症的治疗提供一种高效抑菌、加速愈合的治疗手段。PURPOSE:To investigate the therapeutic efficacy of a new composite gel of carboxymethyl chitosan/β-dextran in rat dry socket model.METHODS:Carboxymethyl chitosan/oatβ-dextran composite gel was prepared,its viscosity,appearance and shape,and stability in oral wet environment were investigated.Staphylococcus aureus was selected for in vitro inhibition test.Blank negative control group,carboxymethyl chitosan/oatβ-dextran composite gel group,and iodoform gauze positive control group were established respectively,and the maxillary first molars were extracted to establish a dry socket model in SD rats,to preliminarily evaluate the healing effect of alveolar fossa wounds.The maxillary bone and gingival fixed decalcification were also taken and histopathologically observed for healing as well as immunofluorescence to detect differences in the expression of inflammatory factors between the groups.SPSS 19.0 software package was used for statistical analysis.RESULTS:The compound gel group had obvious antibacterial effect,which can effectively exert local anti-inflammatory and antibacterial efficacy.Compared with the blank control group,visual observation of wound healing,histopathological observation and immunofluorescence inflammatory factor labeling showed that the composite gel group had significant effect on inflammation inhibition and wound healing,which was comparable to the iodoform gauze group.CONCLUSIONS:Carboxymethyl chitosan/oatβ-dextran composite gel has significant antibacterial and anti-inflammatory effects and can accelerate the healing of dry socket wounds in rats,which is expected to provide an efficient antibacterial and accelerated healing therapeutic measure for the treatment of clinical dry socket.

关 键 词：干槽症 羧甲基壳聚糖 燕麦β葡聚糖 复合凝胶 

分 类 号：R782.1[医药卫生—口腔医学]