高效液相色谱法测定蜂王浆和蜂王浆干粉中羟甲基糠醛含量  

Determination of hydroxymethylfurfural in royal jelly and its dry powder by high performance liquid chromatography

作　　者：周萍 李珊珊 尹志红 吴晓群 李樱红 魏琳 胡福良[2] ZHOU Ping;LI Shan-Shan;YIN Zhi-Hong;WU Xiao-Qun;LI Ying-Hong;WEI Lin;HU Fu-Liang(Hangzhou Biyutian Health Products Co.,Ltd.,Tonglu 311500,China;Animal Science College of Zhejiang University,Hangzhou 310058,China;Tonglu County Market Supervision and Administration Bureau,Tonglu 311500,China;Zhejiang Institute for Food and Drug Control,Hangzhou 310052,China)

机构地区：[1]杭州碧于天保健品有限公司,桐庐311500 [2]浙江大学动物科学学院,杭州310058 [3]桐庐县市场监管局,桐庐311500 [4]浙江省食品药品检验研究院,杭州310052

出　　处：《食品安全质量检测学报》2024年第14期148-154,共7页Journal of Food Safety and Quality

基　　金：2023年第一批推荐性国家标准计划项目(20230012-T-442);国家蜂产业技术体系专项(CARS-44);2021年度浙江省市场监督管理局科技项目(20210127)。

摘　　要：目的建立高效液相色谱法测定蜂王浆和蜂王浆干粉中羟甲基糠醛含量。方法将蜂王浆经氨水碱化,使样液呈中性或者弱碱性,用乙酸乙酯提取,离心,取上清液蒸干后复溶解,过0.22μm滤膜,采用C_(18)色谱柱分离,以羟甲基糠醛为标准品进行外标法定量,以高效液相色谱仪进行检测。结果羟甲基糠醛在20~500μg/kg范围内线性良好,相关系数r2为0.9983,添加回收率为86.1%~100.8%,相对标准偏差为4.6%~8.0%,检出限为6μg/kg,定量限为20μg/kg。对11个样品进行测定,蜂王浆和蜂王浆干粉中羟甲基糠醛含量不等,与储存条件有关。结论本研究方法可用于检测蜂王浆及其冻干粉中羟甲基糠醛的含量,羟甲基糠醛具有评价蜂王浆及其冻干粉新鲜度的潜力。Objective To establish a method for determination of hydroxymethylfurfural in royal jelly and its dry powder by high performance liquid chromatography.Methods Royal jelly was alkalized with ammonia to make the sample neutral or weakly alkaline.The royal jelly sample was extracted using ethyl acetate and then centrifuged.The supernatant was evaporated to dryness and then dissolved.After passed through 0.22μm filter membrane,the resultant sample was separated by C_(18)column and tested using liquid chromatography.The sample was quantified by external standard method as the standard of hydroxymethylfurfural.Results Hydroxymethylfurfural had good linearity in the range of 20–500μg/kg.The correlation coefficients were 0.9983.The recoveries of addition were 86.1%–100.8%,and the relative standard deviation were 4.6%–8.0%.The limit of detection was 6μg/kg and limit of quantitation was 20μg/kg.The contents of hydroxymethylfurfural in 11 samples royal jelly and royal jelly dry powder were different and related to storage conditions.Conclusion This method can be used to determine the content of hydroxymethylfurfural in royal jelly and its freeze-dried powder,and hydroxymethylfurfural has the potential to evaluate the freshness of royal jelly and its freeze-dried powder.

关 键 词：蜂王浆 蜂王浆干粉 羟甲基糠醛 高效液相色谱法 新鲜度 

分 类 号：S896.3[农业科学—特种经济动物饲养] O657.72[农业科学—畜牧兽医]