辽宁省稻瘟病菌群体交配型分布及育性差异  

Differential analysis on mating type distribution and fertility capacity of Magn-porthe oryzae of Liaoning Province

作　　者：闫晗[1] 陈立杰[2] 韩涛 董海[1] 徐晗[1] 褚晋 缪建锟[1] 杨皓[1] 李志强[1] YAN Han;CHEN Lijie;HAN Tao;DONG Hai;XU Han;CHU Jin;MIAO Jiankun;YANG Hao;LI Zhiqiang(Institute of Plant Protection,Liaoning Academy of Agricultural Sciences,Shenyang 11016l,China;College of Plant Protection,Shenyang Agricultural University,Shenyang 110886,China;Donggang Demonstration Breeding Farm,Dandong 118300,China)

机构地区：[1]辽宁省农业科学院植物保护研究所,沈阳110161 [2]沈阳农业大学植物保护学院,沈阳110886 [3]东港市示范繁殖农场,丹东118300

出　　处：《植物病理学报》2024年第4期679-686,共8页Acta Phytopathologica Sinica

基　　金：辽宁省农科院基本业务费计划项目(2021HQ1903);国家自然科学基金(U22A20463)。

摘　　要：稻瘟病菌在田间具有丰富的多态性。在辽宁省5个粳稻主产区采集并分离了336株稻瘟菌单孢菌株,并对其交配型分布和育性能力差异进行了检测,分析稻瘟病菌的变异机制。通过PCR技术检测交配型,发现所有供试菌株均属于单一交配型MAT1-2,群体遗传中出现了显著的交配型偏离现象。采用对峙培养杂交法,与标准菌株P9(MAT1-1)配对检测育性能力,发现所有供试菌株的平均可育性菌株比例为37.50%,平均形成子囊壳38.8个,可育菌株比例整体偏低,存在地域性分布规律,稻瘟病菌群体育性可能受积温影响。本研究首次明确了辽宁省稻瘟菌交配型分布和育性能力地域性差异。鉴于交配型的单一和可育菌株的缺乏,辽宁省粳稻主产区田间流行的稻瘟菌多是无性繁殖构成群体。Rice blast fungus has diverse polymorphism in paddy fields.To clarify the role of genetic recombination on biodiversity of Magnaporthe oryzae population in paddy fields,336 single-spore isolates of M.oryzae were collected from five main rice-producing regions of Liaoning Province,and their difference on mating type distribution and fertility capacity were analyzed.The PCR amplification on mating type genes showed that all 336 isolates belonged to the same mating type,MAT1-2.The confrontation cultivations of all isolates with a standard strain P9 having an opposite mating type(MAT1-1)showed that the average proportion of fertile strains was 37.5%and the average number of perithecia of each cross was 38.8.Moreover,the fertility capacities of the isolates from the five main rice-producing regions were significantly varied.Taken together,these findings suggest that sexual reproduction of M.oryzae population in Liaoning Province is rare or probably non-existent though they retain certain fertility capacities,and the biodiversity of M.oryzae population in paddy fields may be attributed by other factors.

关 键 词：辽宁省 稻瘟病菌 交配型 育性 

分 类 号：S432.44[农业科学—植物病理学]