Microfluidic-based isolation of circulating tumor cells with high-efficiency and high-purity  

作　　者：Feng Wu Xuemin Kong Yixuan Liu Shuli Wang Zhong Chen Xu Hou 

机构地区：[1]Fujian Engineering Research Center for Solid-State Lighting,Department of Electronic Science,School of Electronic Science and Engineering,Xiamen University,Xiamen 361005,China [2]College of Physics and New Energy,Xuzhou University of Technology,Xuzhou 221018,China [3]State Key Laboratory of Physical Chemistry of Solid Surfaces,College of Chemistry and Chemical Engineering,Xiamen University,Xiamen 361005,China [4]College of Physical Science and Technology,Xiamen University,Xiamen 361005,China [5]Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province(IKKEM),Xiamen 361102,China

出　　处：《Chinese Chemical Letters》2024年第8期101-111,共11页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China(Nos.52025132,22005255,21975209,21621091,22021001,T2241022);the National Science Foundation of Fujian Province of China(No.2022J02059);the Fundamental Research Funds for the Central Universities of China(No.20720220085);the 111 Project(Nos.B17027,B16029);the Science and Technology Projects of Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province(No.RD2022070601),the New Cornerstone Science Foundation through the XPLORER PRIZE.

摘　　要：The isolation of circulating tumor cells(CTCs)from complex biological samples is of paramount signifi-cance for advancing cancer diagnosis,prognosis,and treatment.However,the low concentration of CTCs and nonspecific adhesion of white blood cells(WBCs)present challenges that hinder the efficiency and purity of captured CTCs.Microfluidic-based strategies utilize precise fluid control at the micron level to incorporate specific micro/nanostructures or recognition molecules,enabling effective CTCs separation.Moreover,by employing surface modification designs that exhibit exceptional anti-adhesion properties against WBCs,the purity of isolated CTCs can be further enhanced.This review offers an in-depth explo-ration of recent advancements,challenges,and opportunities associated with microfluidic-based CTCs iso-lation from biological samples.Firstly,we will comprehensively introduce the microfluidic-based strate-gies for achieving high-efficiency CTCs isolation,which includes the morphological design of microchan-nels for physical force-based CTCs isolation and the specific modification of microchannel surfaces for affinity-based CTCs isolation.Subsequently,a review of recent research advances in microfluidic-based high-purity CTCs isolation is presented,focusing on strategies that decrease the nonspecific adhesion of WBCs through surface micro-/nanostructure construction or chemical and biological modification.Finally,we will summarize the article by providing the prospective opportunities and challenges for the future development of microfluidic-based CTCs isolation.

关 键 词：MICROFLUIDICS ISOLATION Circulating tumor cells High-efficiency HIGH-PURITY 

分 类 号：R730[医药卫生—肿瘤]