Enzymatically controlled DNA tetrahedron nanoprobes for specific imaging of ATP in tumor  

作　　者：Xiaohong Wen Mei Yang Lie Li Mingmin Huang Wei Cui Suping Li Haiyan Chen Chen Li Qiuping Guo 

机构地区：[1]College of Biology,State Key Laboratory of Chemo/Biosensing and Chemometrics,College of Chemistry and Chemical Engineering,Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province,Hunan University,Changsha 410082,China

出　　处：《Chinese Chemical Letters》2024年第8期221-224,共4页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China(Nos.21877030,21735002,21778016).

摘　　要：Intracellular ATP is an emerging biomarker for cancer early diagnosis because it is a key messenger for regulating the proliferation and migration of cancer cells.However,the conventional ATP biosensing strat-egy is often limited by the undesired on-target off-tumor interference.Here,we reported a novel strategy to design enzymatically controlled DNA tetrahedron nanoprobes(En-DT)for biosensing and imaging ATP in tumor cells.The En-DT was designed via rational engineering of structure-switching aptamers with the incorporation of an enzyme-activatable site and further conjugation on the DNA tetrahedron.The En-DT could be catalytically activated by apurinic/apyrimidinic endonuclease 1(APE1)in cancer cells,but they did not respond to ATP in normal cells,thereby enabling cancer-specific ATP biosensing and imaging in vitro and in vivo with improved tumor specificity.This strategy would facilitate the precise detection of a broad range of biomarker in tumors and may promote the development of smart probes for cancer diagnosis.

关 键 词：Enzyme-controlled APE1 DNA tetrahedron Cancer-specific ATP biosensing 

分 类 号：R730.4[医药卫生—肿瘤] O657.3[医药卫生—临床医学]