我国玉米大斑病菌生理小种及交配型组成分析  

Analysis of Physiological Race and Mating Type Composition of Setosphaeria turcica in China

作　　者：马周杰 黄宇飞[2] 温胜慧 杨俊伟 贾鑫 赵变平 李彦良 高增贵[2] 王建军[1] MA Zhou-jie;HUANG Yu-fei;WEN Sheng-hui;YANG Jun-wei;JIA Xin;ZHAO Bian-ping;LI Yan-liang;GAO Zeng-gui;WANG Jian-jun(Maize Research Institute,Shanxi Agricultural University/Xinzhou Observation and Experiment Station of National Plant Protection,Xinzhou 034000;Plant Protection College,Shenyang Agricultural University,Shenyang 110866,China)

机构地区：[1]山西农业大学玉米研究所/国家植物保护忻州观测实验站,山西忻州034000 [2]沈阳农业大学植物保护学院,沈阳110866

出　　处：《玉米科学》2024年第5期105-111,共7页Journal of Maize Sciences

基　　金：国家重点研发计划课题项目(2018YFD0300307,2017YFD0300704,2016YFD0300704)、山西农业大学生物育种工程项目(YZGC064)、山西省玉米种业创新良种联合攻关项目。

摘　　要：利用Ht单基因鉴别寄主对2021年采自我国10个省(自治区)57个市县的127株玉米大斑病菌进行生理小种鉴定,采用特异性引物对供试菌株进行交配型检测。生理小种鉴定结果显示,我国玉米大斑病菌主要包括0、1、2、12、3、13、23、N、1N、2N、3N、13N、23N、123N等14个生理小种,其中,0号和1号生理小种分别占供试菌株的34.65%和28.35%,为主要小种类群。我国玉米大斑病菌生理小种存在多元化,东北地区生理分化更为明显。供试菌株对Ht1、Ht2、Ht3、HtN的毒性频率分别为41.73%、9.45%、25.20%、17.32%。交配型检测结果显示,分离株中共有A、a两种交配型,且a交配型比例明显高于A交配型,不同省份玉米大斑病菌交配型组成情况存在差异。The physiological races of 127 isolates of Setosphaeria turcica collected from 57 cities and counties of 10 provinces(autonomous regions)in 2021 were identified on differential hosts containing Ht single resistance gene,and the mating type of the tested isolates was detected by specific primers.The identification results of physiological races showed that there were 14 physiological races including 0,1,2,12,3,13,23,N,1N,2N,3N,13N,23N,123N.Among these races,race 0 and race 1 accounted for 34.65% and 28.35% of the tested isolates respectively,and were the main race group.There was a diversity of physiological races of S.turcica in China,with more obvious physiological differentiation in Northeast China.The toxicity frequency of the tested isolates to Ht1,Ht2,Ht3 and HtN was 41.73%,9.45%,25.20% and 17.32%,respectively.The results of mating type detection showed that there were two mating types,A and a,in the isolates,and the proportion of mating type a was significantly higher than that of mating type A,and the composition of mating types varied in different provinces.

关 键 词：玉米 玉米大斑病菌 生理小种 毒力分析 交配型 

分 类 号：S435.131[农业科学—农业昆虫与害虫防治]