ERβ结合TGFBR1启动子序列促进前列腺癌细胞增殖和凋亡  

ERβpromotes TGFBR1 expression,enhancing the proliferation and apoptosis in prostate cancer cells

作　　者：伍宏亮 汪盛 陈志军 杨帅 孙文衍 关翰 WU Hongliang;WANG Sheng;CHEN Zhijun;YANG Shuai;SUN Wenyan;GUAN Han(Department of Urology,The First Affiliated Hospital of Bengbu Medical University,Bengbu 233000,Anhui,China)

机构地区：[1]蚌埠医科大学第一附属医院泌尿外科,安徽蚌埠233000

出　　处：《右江民族医学院学报》2024年第4期474-479,共6页Journal of Youjiang Medical University for Nationalities

基　　金：安徽省高校自然科学重点项目(2023AH051942);蚌埠市级科技创新指导类项目(20210341)。

摘　　要：目的探讨雌激素受体β(estrogen receptorβ,ERβ)如何结合生长因子β受体1(transforming growth factor beta receptor 1,TGFBR1)启动子序列进而促进前列腺癌进展。方法收集前列腺癌患者样本,通过免疫组化检测TGFBR1的表达,构建沉默TGFBR1载体后,细胞克隆实验、CCK-8实验、EDU实验、细胞凋亡实验检测沉默TGFBR1对前列腺癌增殖和转移能力的影响,双荧光素酶报告基因实验检测ERβ是否结合TGFBR1,PCR和Western Blot检测干扰ERβ后对TGFBR1表达的影响。结果TGFBR1在前列腺癌中过表达,且TGFBR1高表达与较差的预后直接相关。细胞克隆实验、CCK-8实验、细胞凋亡实验表明,相较于对照组(NC),沉默TGFBR1组的细胞增殖能力显著抑制,而凋亡增多,而oe-ERβ+siTGFBR1组的细胞增殖、凋亡能力与NC组差异不明显。双荧光素酶报告基因实验显示,ERβ结合TGFBR1的启动子序列,且沉默ERβ后,抑制TGFBR1的表达。结论ERβ通过结合TGFBR1启动子序列进而促进前列腺癌细胞增殖和凋亡。Objective To explore how estrogen receptorβ(ERβ)regulates Transforming Growth Factor Beta Receptor 1(TGFBR1)to promote the progression of prostate cancer.Methods Samples of prostate cancer patients were collected,and the expression of TGFBR1 was detected using immunohistochemistry.A silencing TGFBR1 vector was constructed to examine its effects on the proliferation and metastasis of prostate cancer.Various assays were employed,including cell cloning,CCK-8,EDU and apoptosis assays.A dual-luciferase reporter gene assay was used to detect whether ERβbinds to TGFBR1.PCR and Western blot analyses were performed to assess the impact of ERβinterference on TGFBR1 expression.Results TGFBR1 was found to be overexpressed in prostate cancer tissue,with high expression levels directly related to poor prognosis.Compared with the control group(NC),Cell cloning,CCK-8 and apoptosisassays showed that cell proliferation and apoptosis of TGFBR1 group were significantly inhibited,while cell proliferation and apoptosis of Oe-ER-β+siTGFBR1 group were not significantly different from those of NC group.Dual luciferase reporter gene assay revealed that ERβbinds to the TGFBR1 promoter sequence.Moreover,silencing ERβled to a decrease in TGFBR1 expression.Conclusion ERβcan promote the progression of prostate cancer by binding to TGFBR1 promoter sequence,thereby enhancing cell proliferation and apoptosis.

关 键 词：受体 转化生长因子β 雌激素受体Β 前列腺肿瘤 增殖 凋亡 

分 类 号：R737.25[医药卫生—肿瘤]