基于Box-Behnken设计-响应面法和反向传播神经网络优化核桃青皮萘醌的提取工艺  

Extraction process optimization of naphthoquinone from Walnut green husk based on Box-Behnken design-response surface methodology and back propagation neural network

作　　者：高洁 马婧怡 GAO Jie;MA Jingyi(Department of Pharmacy,The Fifth Affiliated Hospital of Xinjiang Medical University,Urumqi 830000,China;Office of Drug Clinical Trial Institutions,Zhuhai People's Hospital(ZhuHai Clinical Medical College of Jinan University),Zhuhai 519000,Guangdong Province,China)

机构地区：[1]新疆医科大学第五附属医院药学部,乌鲁木齐830000 [2]珠海市人民医院(暨南大学珠海临床医学院)药物临床试验机构办公室,广东珠海519000

出　　处：《中国药师》2024年第8期1286-1295,共10页China Pharmacist

基　　金：新疆维吾尔自治区自然科学基金项目(2021D01C442)。

摘　　要：目的在以乙醇体积分数、料液比、提取温度为影响因素的单因素试验基础上,优化核桃青皮萘醌的提取工艺,并考察核桃青皮萘醌提取物体外抗氧化活性。方法采用醇提取法,应用Box-Behnken设计-响应面法和反向传播神经网络模拟优化提取工艺。测定核桃青皮萘醌提取物对羟自由基(HO·)、超氧阴离子自由基(O^(2-)·)和1,1-二苯基-2-三硝基苯肼自由基(DPPH·)的清除能力。结果最佳提取条件为乙醇体积分数为40%、料液比为1∶30(g/mL)、提取温度70℃、提取时间2 h,得到萘醌提取量(20.182±1.851)mg/g。质量浓度0.1 g/L的核桃青皮萘醌提取物溶液对HO·、O^(2-)·和DPPH·的清除率分别为16.09%、67.28%和73.00%。结论优化后的核桃青皮萘醌提取工艺稳定、可行。核桃青皮萘醌提取物表现出较好的抗氧化活性。Objective To optimize the extraction process of naphthoquinone from Walnut green husk with ethanol volume fraction,solid-liquid ratio and extraction temperature as the influencing factors on the basis of a single factor test,and study the in vitro antioxidant activity of the extract.Methods The alcohol extraction method was used to extract naphthoquinone from Walnut green husk,and extraction process was optimized by Box-Behnken design-response surface methodology and back propagation neural network simulation.The scavenging capacity of naphthoquinone from Walnut green husk on hydroxyl radical(HO·),superoxide anion radical(O^(2-)·)and 1,1-diphenyl-2-trinitrophenylhydrazine radical(DPPH·)were determined.Results The optimal extraction condition was as follows:ethanol volume fraction of 40%,solid-liquid ratio of 1∶30(g/mL),extraction temperature of 70℃,extraction time of 2 h,and the extraction amount of naphthoquinone obtained was(20.182±1.851)mg/g.The scavenging rates of 0.1 g/L naphthoquinone extract on HO·,O^(2-)·and DPPH·were 16.09%,67.28%and 73.00%,respectively.Conclusion The extraction process of naphthoquinone from Walnut green husk is stable and feasible.The naphthoquinone extract from Walnut green husk exhibits good antioxidant activity.

关 键 词：核桃青皮 萘醌 提取工艺优化 Box-Behnken设计-响应面法 神经网络 体外抗氧化 

分 类 号：R284.2[医药卫生—中药学]