臭椿铁屎米-6-酮生物碱对黄瓜枯萎病菌的抑制作用  

Inhibitory Effect of Canthin-6-one Isolated from Ailanthus altissima against Fusarium oxysporum f.sp.cucumerinum

作　　者：李永春 赵美荣 肖金洋 张智[1] LI Yongchun;ZHAO Meirong;XIAO Jinyang;ZHANG Zhi(School of Forestry,Northeast Forestry University,Harbin 150040,China;College of Chemistryand Life Science,Chifeng University,Chifeng 024000,China)

机构地区：[1]东北林业大学林学院,哈尔滨150040 [2]赤峰学院化学与生命科学学院,赤峰024000

出　　处：《吉林农业大学学报》2024年第4期560-565,共6页Journal of Jilin Agricultural University

基　　金：内蒙古自治区高等学校科学研究项目(NJZY19215)。

摘　　要：为探索臭椿铁屎米-6-酮生物碱在植物病害防治方面的应用前景,以黄瓜枯萎病菌为供试菌,采用微量稀释法、菌丝生长速率法和载玻片萌发法研究其抑菌活性。通过测定铁屎米-6-酮处理前后黄瓜枯萎病菌的相对电导率、丙二醛和过氧化氢含量以及保护酶活性,从细胞膜角度分析铁屎米-6-酮抑菌作用机制。结果表明:铁屎米-6-酮对黄瓜枯萎病菌有较强的抑菌作用,最小抑菌浓度为32μg/mL,对菌丝生长和孢子萌发抑制作用明显,EC;50;分别为4.2μg/mL和14.6μg/mL。铁屎米-6-酮通过诱导黄瓜枯萎病病原菌丙二醛和过氧化氢含量增加,影响氧化系统的平衡,破坏细胞膜完整性,从而抑制病原菌菌丝生长。The purpose of this study is to explore the application prospects of canthin-6-one iso⁃lated from Ailanthus altissima in controlling plant diseases.Micro-well dilution,mycelial growth rate and spore germination test were used to evaluate the inhibitory effect of canthin-6-one on Fusarium oxysporum f.sp.cucumerinum.The anti-fungal mechanism of canthin-6-one was analyzed by detect⁃ing the relative conductivity,the content of MDA and H2O2,and the activity of protective enzymes.The results showed that canthin-6-one exhibited excellent competences for suppressing the growth of the pathogens with the MIC of 32μg/mL.This compound could obviously inhibit the growth of pathogenic mycelium and spore germination,and the EC50 value was 4.2μg/mL and 14.6μg/mL,re⁃spectively.In addition,the content of MDA and H2O2 increased significantly after treatment with can⁃thin-6-one,which disturbed the dynamic balance.The integrity of the membrane system was dam⁃aged,resulting in inhibition of mycelial growth.

关 键 词：臭椿 铁屎米-6-酮 生物碱 黄瓜枯萎病菌 抑菌活性 

分 类 号：S436.421.1[农业科学—农业昆虫与害虫防治]