低强度超声和不同辐照时间组合激励微泡空化对裸鼠肾癌移植瘤血流灌注的影响  

Effects of low intensity ultrasound combined irradiation time to stimulate microbubble cavitation on renal carcinoma perfusion in nude mice

作　　者：王娟[1] 罗婷婷 姚雷 马玉林[1] 张帆[1] 王文刚[1] 王岳恒[3] 剧红娟[1] WANG Juan;LUO Tingting;YAO Lei;MA Yulin;ZHANG Fan;WANG Wengang;WANG Yueheng;JU Hongjuan(Department of Abdominal Ultrasound,The Second Hospital of Hebei Medical University,Hebei,Shijiazhuang 050000,China;Department of Ultrasound,the Second Affiliated Hespital of Army Medical University,Chongqing 400037,China;Department of Cardiac Ultrasound,The Second Hospital of Hebei Medical University,Hebei,Shijiazhuang 050000,China)

机构地区：[1]河北医科大学第二医院腹部超声科,河北石家庄050000 [2]陆军军医大学第二附属医院超声科,重庆400037 [3]河北医科大学第二医院心脏超声科,河北石家庄050000

出　　处：《石河子大学学报（自然科学版）》2024年第4期464-470,共7页Journal of Shihezi University(Natural Science)

基　　金：河北省医学科学研究课题计划项目(20230468)。

摘　　要：目的探讨不同辐照时长下低强度超声激励微泡空化对裸鼠肾癌移植瘤血流灌注的影响。方法选用雄性BALB/c裸鼠30只,于腋下建立786-O人肾透明细胞癌肿瘤模型,根据空化治疗时间(T)随机分为3组,分别为A组(T=20 min)、B组(T=10 min)、C组(T=5 min),每组10只。于治疗前和治疗后即刻、4 h、6 h分别进行造影检查(CEUS)及微血流显像(MFI);组织学染色检测空化治疗结束后肿瘤微血管状态。结果与治疗前相比,A组治疗后即刻肿瘤内血流灌注增加,PI、AUC的值分别增长7.4%(P<0.05)、10.6%(P<0.05);治疗后4 h、6 h肿瘤血流灌注比治疗后即刻显著降低(均P<0.05)。B组结果显示,与治疗前相比,治疗后即刻、治疗后4 h与治疗后6 h的PI数值分别增长了8.1%、15.1%和3.1%,且相应的AUC数值增长率为18.7%、31.2%和9.3%。方差分析结果显示各组PI、AUC值存在显著差异(均P<0.01),且组间两两比较PI、AUC差异均有统计学意义(均P<0.01);C组结果显示,治疗后即刻、治疗后4 h、6 h肿瘤内血流灌注与治疗前比较均降低。肿瘤内血流灌注PI、AUC值均显著减小,与治疗前相比差异有统计学意义(均P<0.05)。MFI显像各组视觉评分差异分析结果与造影分析结论一致。病理图显示:B组可见较明显的血管扩张,而C组可见管腔受压变窄、出血,微血管密度减低(P<0.01)。结论低强度超声激励微泡空化治疗时间影响肿瘤血流灌注,在本研究中治疗时间为10 min时产生的血流增强效应最优。Objective To explore the effect of low-intensity ultrasound-induced microbubble cavitation on blood perfusion of renal carcinoma in nude mice under different irradiation durations.Methods Thirty male BALB/c nude mice were selected to establish a 786-O tumor model in the armpit.They were randomly divided into 3 groups according to the cavitation treatment time(T),namely group A(T=20 min)and group B(T=10 min),group C(T=5 min),10 animals in each group.Contrast imaging(CEUS)and microflow imaging(MFI)were performed pre-treatment and immediately,4 h,and 6 h after treatment;histological staining was performed to detect the microvascular status of the tumor after treatment.Results Compared with before treatment,the intratumoral blood perfusion in Group A increased immediately after treatment,and the PI and AUC values increased by 7.4%(P<0.05)and 10.6%(P<0.05)respectively;Tumor blood perfusion was significantly lower at 4 h and 6 h comparing to immediately after treatment(all P<0.05).The results of Group B showed that compared with pre-treatment,the PI values immediately,4 hours and 6 hours after treatment increased by 8.1%,15.1%and 3.1%respectively,and the corresponding AUC value growth rates were 18.7%and 31.2%.and 9.3%.The results of analysis of variance showed that there were significant differences in the PI and AUC values of each group(both P<0.01),and the differences in PI and AUC between the groups were statistically significant(both P<0.01);the results of group C showed that immediately after treatment,the blood perfusion in the tumor decreased 4 h and 6 h after treatment compared with pre-treatment.The intratumoral blood perfusion PI and AUC values were significantly reduced,and the differences were statistically significant compared with those before treatment(both P<0.05).The results of the difference analysis of visual scores among each group under MFI ima-ging were consistent with the conclusions of the contrast analysis.The pathological pictures showed that obvious blood vessel dilation was seen in group B,

关 键 词：超声 微气泡 786-O人肾透明细胞癌 微血流成像 血管组织 

分 类 号：R730.59[医药卫生—肿瘤]