L-缬氨酸发酵液活性炭脱色工艺优化研究  

Study on Optimization of Activated Carbon Decolorization Process of L-valine Fermentation Broth

作　　者：房夕杰 李天罡 王松江 郭传庄 王建彬 李小双 王硕 宋佳 王长海 连战 FANG Xijie;LI Tiangang;WANG Songjiang;GUO Chuanzhuang;WANG Jianbin;LI Xiaoshuang;WANG Shuo;SONG Jia;WANG Changhai;LIAN Zhan(Dongxiao Biotechnology Co.,Ltd.,Weifang 262218,China)

机构地区：[1]东晓生物科技股份有限公司,潍坊262218

出　　处：《食品与发酵科技》2024年第4期20-24,共5页Food and Fermentation Science & Technology

基　　金：国家重点研发计划资助(2023YFD1300700)。

摘　　要：该文对经大肠杆菌发酵得到L-缬氨酸发酵液进行初步膜过滤,后使用活性炭进行脱色,以脱色率和L-缬氨酸保留率评价脱色效果。考察了4种不同型号的活性炭对L-缬氨酸的脱色效果,实验表明,选择元力HPC-07活性炭为L-缬氨酸脱色活性炭,脱色条件为活性炭用量2%、脱色温度65℃、发酵液pH 5.5、脱色时间30min。发酵液颜色由棕黄色变为澄清无色,且L-缬氨酸保留率高于98.0%,上述研究为企业生产高质量L-缬氨酸提供数据参考。In this paper,the L-valine fermentation broth obtained by Escherichia coli fermentation was preliminarily filtered by membrane filtration,followed by decolorization using activated carbon,and the decolorization effect was evaluated by decolorization rate and L-valine retention rate.The decolorization effect of four different types of activated carbon on L-valine was investigated,and the experiment determined that the Yuanli HPC-07 activated carbon was selected as the decolorizing activated carbon,and the decolorization conditions were activated carbon dosage of 2%,decolorization temperature 65℃,fermentation broth pH 5.5,and decolorization time of 30 min.The color of the fermentation liquid was changed from brownish-yellow to a clarified colorless,and the retaining rate of L-valine was higher than 98.0%.The above research provides data reference for enterprises to produce high-quality L-valine.

关 键 词：L-缬氨酸 脱色工艺 活性炭 

分 类 号：TS202.3[轻工技术与工程—食品科学]