定量代谢组学比较分析蕾薹期油菜不同部位营养差异  

Comparative Quantitative Metabolomics Analysis of Nutritional Differences in Different Plant Parts of Oilseed Rape(Brassica napus L.)at the Bud Stage

作　　者：吴秋雨 林红 吴宗远 从艳霞 张维农 WU Qiuyu;LIN Hong;WU Zongyuan;CONG Yanxia;ZHANG Weinong(School of Food Science and Engineering,Wuhan Polytechnic University,Wuhan 430023,China;Key Laboratory for Deep Processing of Major Grain and Oil,Ministry of Education,Wuhan 430023,China;Hubei Oils and Fats Fine Chemical Engineering and Technology Research Center,Wuhan 430023,China)

机构地区：[1]武汉轻工大学食品科学与工程学院,湖北武汉430023 [2]大宗粮油精深加工教育部重点实验室,湖北武汉430023 [3]湖北省油脂精细化工工程技术研究中心,湖北武汉430023

出　　处：《食品科学》2024年第18期152-159,共8页Food Science

基　　金：“十四五”国家重点研发计划重点专项(2021YFD1600500)。

摘　　要：本研究采用定量核磁共振(nuclear magnetic resonance,NMR)与高效液相色谱-串联质谱(high performance liquid chromatography-tandem mass spectrometry,HPLC-MS/MS)技术分析大地95油菜蕾薹期不同部位(茎、叶、花苞)小分子营养物的组成差异。结果表明,通过1H-NMR与HPLC-MS/MS分析在大地95菜薹茎、叶、花苞中共定性定量得到53种小分子化合物,其中具有显著差异的物质有亮氨酸、异亮氨酸、缬氨酸、γ-氨基丁酸、丙氨酸、脯氨酸、苯丙氨酸、乳酸、柠檬酸、琥珀酰胺、β-D-葡萄糖、α-D-葡萄糖、蔗糖、果糖、胆碱、甜菜碱、延胡索酸、黄芩素、阿魏酸、山柰酚、芥子碱、反式肉桂酸、橙皮素。糖类物质在油菜茎部的含量最高;而胆碱、琥珀酰胺、多数氨基酸、有机酸以及酚酸类物质主要在花苞中积累,叶部的优势代谢物以黄岑素、芥子碱和柠檬酸为主。通过分析代谢通路发现这些差异代谢物主要涉及植物的柠檬酸盐循环,黄酮和黄酮醇的生物合成,苯丙氨酸代谢,氨酰基-tRNA生物合成,缬氨酸、亮氨酸和异亮氨酸的生物合成,糖酵解/糖异生,苯丙氨酸、酪氨酸和色氨酸的生物合成等。本研究结果可为全面评估蕾薹期油菜的营养价值提供数据参考,并为选育营养丰富的油菜品种提供科学依据。In this study,quantitative nuclear magnetic resonance(NMR)spectroscopy and high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)were used to analyze the differences in the composition of small-molecule nutrients in different parts(stems,leaves and flower buds)of‘Dadi 95’oilseed rape(Brassica napus L.)at the bud stage.The results showed that 53 small-molecule compounds were identified and quantified in total.Among these,substances with significant differences were leucine,isoleucine,valine,γ-aminobutyric acid,alanine,proline,phenylalanine,lactate,citrate,succinamide,β-D-glucose,α-D-glucose,sucrose,fructose,choline,betaine,fumaric acid,baicalein,ferulic acid,kaempferol,sinapine,trans-cinnamic acid,and hesperetin.Comparative analysis revealed that the content of carbohydrate was the highest in the stem of B.napus L.,whereas choline,succinamide,most of amino acids,organic acids,and phenolic acids were mainly accumulated in the buds,and the dominant metabolites in the leaves were baicalein,sinapine,and citric acid.Metabolic pathway analysis demonstrated that these differential metabolites were mainly involved in the citrate cycle,flavone and flavanol biosynthesis,phenylalanine metabolism,aminoacyl-tRNA biosynthesis,valine,leucine and isoleucine biosynthesis,glycolysis/gluconeogenesis,and phenylalanine,tyrosine and tryptophan biosynthesis in the plant.The results from this study provide foundational data for comprehensively assessing the nutritional value of B.napus L.at the bud stage,and a scientific basis for selecting and breeding nutritious oilseed rape varieties.

关 键 词：油菜 蕾薹期 代谢组学 定量核磁共振 高效液相色谱-串联质谱 

分 类 号：TS222.1[轻工技术与工程—粮食、油脂及植物蛋白工程]