基于甲基化特异性PCR的外周血循环游离DNA甲基化检测对乳腺癌诊断价值的荟萃分析  

Diagnostic value of circulating cell-free DNA methylation detected based on methylation-specific PCR for breast cancer:a Meta-analysis

作　　者：刘杰 朱启富 张丹 余琦慧 郑欣 姚水洪[1] 汪新华 LIU Jie;ZHU Qifu;ZHANG Dan;YU Qihui;ZHENG Xin;YAO Shuihong;WANG Xinhua(Medical School,Quzhou College of Technology,Quzhou,Zhejiang,324000,China;Kaihua County Yinkeng Rural Hospital,Quzhou,Zhejiang,324309,China)

机构地区：[1]衢州职业技术学院医学院,浙江衢州324000 [2]开化县音坑乡卫生院,浙江衢州324309

出　　处：《当代医学》2024年第13期1-5,共5页Contemporary Medicine

基　　金：衢州市科技特派员项目(2022T026)。

摘　　要：目的通过Meta分析系统评估基于甲基化特异性PCR(MSP)的外周血循环游离DNA(cfDNA)甲基化检测对乳腺癌的诊断价值。方法检索PubMed、Embase、Cochrane数据库,检索时间为2011年1月至2021年12月,以“Breast neoplasms”“Breast cancer”“Methylation”“Cell-free DNA”等为检索词,检索有关血液循环cfDNA甲基化用于乳腺癌诊断的文献,根据纳入及排除标准筛选文献。使用QUADAS-2对纳入文献进行质量评价,提取研究数据并使用Stata 16.0对各效应量进行合并,分析异质性及来源,以Deek’s法检验发表偏倚。结果共检索318篇文献,最终纳入12篇文献的27项研究进行Meta分析,患者2195例,纳入研究存在高度异质性(I^(2)>50.00%)。采用随机效应模型合并灵敏度为0.43[95%CI(0.31~0.56)],合并特异度为0.97[95%CI(0.94~0.99)],合并阳性似然比(LR+)为16.30[95%CI(7.00~37.80)],合并阴性似然比(LR-)为0.59[95%CI(0.47~0.73)],合并诊断比值比(DOR)为28.00[95%CI(11.00~70.00)],合并AUC为0.85[95%CI(0.82~0.88)]。结论基于MSP的外周血cfDNA甲基化检测对乳腺癌有较高的辅助诊断价值。Objective To evaluate the diagnostic value of circulating cell-free DNA(cfDNA)methylation detected based on methylation-specific PCR(MSP)for breast cancer by Meta-analysis.Methods PubMed,Embase,Cochrane databases were searched from January 2011 to December 2021,"Breast neoplasms""Breast cancer""Methylation"and"Cell-free DNA"were used as the search words to search the literature on the diagnosis of breast cancer using cfDNA methylation,and the literature was screened according to the inclusion and exclusion criteria.QUADAS-2 was used to evaluate the quality of the included articles,the research data were extracted and the effect sizes were pooled using Stata 16.0 to analyze the heterogeneity and sources,and the publication bias was tested by Deek's method.Results A total of 318 literatures were retrieved,and 27 studies of 12 literatures were included for Meta-analysis,involving 2195 patients,there was high heterogeneity among the included studies(I^(2)>50.00%).Using random effect model,the pooled sensitivity,specificity,positive likelihood ratio(LR+),negative likelihood ratio(LR-),diagnostic odds ratios(DOR)and AUC were 0.43(95%CI[0.31-0.56]),0.97(95%CI[0.94-0.99]),16.30(95%CI[7.00-37.80]),0.59(95%CI[0.47-0.73])and 28.00(95%CI[11.00-70.00]),and 0.85(95%CI[0.82-0.88]),respectively.Conclusion The detection of cfDNA methylation based on MSP has high auxiliary diagnostic value for breast cancer.

关 键 词：循环游离DNA 甲基化 乳腺癌 甲基化特异性PCR 荟萃分析 

分 类 号：R737.9[医药卫生—肿瘤]