金水宝对去卵巢小鼠骨质疏松的保护作用及其机制  

Protective effect and mechanism of Jinshuibao on osteoporosis in ovariectomized mice

作　　者：刘海霞 朱如愿 马嘉轶 鲁欣悦 赵雨坤 张晓乐 张治国[1] 陈彦静[1] LIU Haixia;ZHU Ruyuan;MA Jiayi;LU Xinyue;ZHAO Yukun;ZHANG Xiaole;ZHANG Zhiguo;CHEN Yanjing(Institute of Basic Theory for Chinese Medicine,China Academy of Chinese Medical Sciences,Beijing 100700,China;Shaanxi Provincial Hospital of Chinese Medicine,Xi’an 710003,Shaanxi,China)

机构地区：[1]中国中医科学院中医基础理论研究所,北京100700 [2]陕西省中医医院,陕西西安710003

出　　处：《现代中西医结合杂志》2024年第13期1753-1756,1893,共5页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：中央级公益性科研院所基本科研业务费专项(YZX-202239,ZZ15-YQ-051)。

摘　　要：目的探讨金水宝对去卵巢小鼠骨质疏松的保护作用及其机制。方法将30只雌性C57BL/6J小鼠随机分为对照组、模型组、金水宝组,每组10只。模型组和金水宝组小鼠采用去卵巢方法构建绝经后骨质疏松症模型,造模第10天开始,金水宝组给予金水宝悬液386 mg/kg灌胃,对照组和模型组给予等体积双蒸水灌胃,均1次/d,每周连续灌胃6 d,连续灌胃15周。采用micro-CT检测小鼠的股骨微结构(骨体积分数、骨小梁数量、骨小梁分离度)及骨密度,采用ELISA法测定小鼠血清I型胶原C端肽(CTX-I)、I型原胶原N端前肽(PINP)、骨碱性磷酸酶(BALP)和抗酒石酸酸性磷酸酶(TRAP)水平,采用Western blot法检测小鼠骨组织中低氧诱导因子-2α(HIF-2α)和肿瘤坏死因子受体相关因子6(TRAF6)的蛋白表达情况。结果与对照组比较,模型组小鼠股骨微结构破坏,骨体积分数、骨小梁数量、骨密度均明显降低(P均<0.05),骨小梁分离度及血清CTX-I、PINP、BALP、TRAP水平和骨组织中HIF-2α、TRAF6蛋白相对表达量均明显升高(P均<0.05);与模型组比较,金水宝组小鼠股骨微结构破坏减轻,骨体积分数、骨小梁数量、骨密度均明显升高(P均<0.05),骨小梁分离度及血清CTX-I、PINP、BALP、TRAP水平和骨组织中HIF-2α、TRAF6蛋白相对表达量均明显降低(P均<0.05)。结论金水宝可有效改善去卵巢小鼠骨质疏松,机制可能与抑制HIF-2α/TRAF6信号通路,调节骨代谢相关。Objective It is to investigate the protective effect and mechanism of Jinshuibao on osteoporosis in ovariectomized mice.Methods Thirty female C57BL/6J mice were randomly divided into control group,model group and Jinshuibao group,with 10 mice in each group.The mice of model group and Jinshuibao group were treated with ovariectomy to establish models of postmenopausal osteoporosis.Starting from the 10th day of modeling,the Jinshuibao group was given Jinshuibao suspension 386 mg/kg by gavage,while the control group and model group were given equal volumes of double distilled water by gavage,all once daily,for 6 consecutive days per week,and continuously gavaged for 15 weeks.The femur bone microstructure(percent bone volume,trabecular number,trabecular separation)and bone mineral density of the mice was detected by Micro-CT,the serum levels of C-terminal telopeptide of type I collagen(CTX-I),N-terminal propeptide of type I procollagen(PINP),bone alkaline phosphatase(BALP)and tartrate-resistant acid phosphatase(TRAP)of the mice were measured by ELISA,and the protein expressions of hypoxia-inducible factor-2α(HIF-2α)and tumor necrosis factor receptor associated factor 6(TRAF6)in bone tissue of the mice were detected by Western blot.Results Compared with the control group,the femur bone microstructure of the mice of the model group was disrupted,and the percent bone volume,trabecular number and bone mineral density were significantly reduced(all P<0.05),the trabecular separation,serum levels of CTX-I,PINP,BALP,TRAP,and relative protein expressions of HIF-2αand TRAF6 in bone tissue were significantly increased(all P<0.05).Compared with the model group,the damage of the femur bone microstructure of the mice of Jinshuibao group was significantly improved,and the percent bone volume,trabecular number and bone mineral density were significantly increased(all P<0.05),the trabecular separation,serum levels of CTX-I,PINP,BALP,TRAP,and relative protein expressions of HIF-2αand TRAF6 in bone tissue were significantly decreas

关 键 词：金水宝 骨质疏松症 去卵巢 低氧诱导因子-2α 肿瘤坏死因子受体相关因子6 骨代谢 

分 类 号：R-332[医药卫生]