培元抗癌汤通过circ_SLIT3/NF-κB轴调节自噬抑制肝癌小鼠肿瘤生长的研究  

Decoction for cultivating primordium and resisting cancer inhibits tumor growth in hepatocellular carcinoma mice by regulating autophagy through circ_SLIT3/NF-κB axis

作　　者：程芙蓉 雷婧[1] 范新宇 张洁[1] 司海龙[1] CHENG Furong;LEI Jing;FAN Xinyu;ZHANG Jie;SI Hailong(Shaanxi University of Chinese Medicine,Xianyang 712000,Shaanxi,China)

机构地区：[1]陕西中医药大学,陕西咸阳712000

出　　处：《现代中西医结合杂志》2024年第13期1801-1806,共6页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：陕西省自然科学基础研究计划项目(2022JM-448)。

摘　　要：目的观察培元抗癌汤对Huh7肝癌小鼠肿瘤生长的抑制作用及对circRNA狭缝引导配体3(circ_SLIT3)/核因子-κB(NF-κB)轴和肿瘤细胞自噬的影响。方法选择12只雄性C57BL/6小鼠,采用右侧腋窝皮下接种肝癌Huh7细胞悬液的方法建立肝癌模型。造模成功后将小鼠随机分为模型组和培元抗癌汤组,分别给予生理盐水和2 g/mL培元抗癌汤灌胃。连续干预11 d后处死小鼠,根据肿瘤的长度和宽度计算肿瘤体积,剥离肿瘤组织称重并计算抑瘤率,透射电镜观察肿瘤组织中自噬小体和自噬泡的形成情况,Western blot法检测肿瘤组织中微管相关蛋白1轻链3(LC3)、半胱氨酸天冬氨酸特异性蛋白酶-3(Caspase-3)、B细胞淋巴瘤/白血病-2蛋白(Bcl-2)、Bcl-2相关X蛋白(Bax)表达情况,PCR法检测肿瘤组织中circ_SLIT3 mRNA表达情况。结果与模型组比较,培元抗癌汤组小鼠的精神状态、纳食、饮水状态等一般情况较好,小鼠肿瘤体积、肿瘤重量均明显降低(P均<0.05),肿瘤细胞中自噬小体、自噬泡明显增多;肿瘤组织中LC3Ⅱ/LC3Ⅰ比值及Caspase-3、Bax、NF-κB蛋白相对表达量均明显升高(P均<0.05),Bcl-2蛋白和circ_SLIT3 mRNA相对表达量均明显降低(P均<0.05)。结论培元抗癌汤能抑制Huh7肝癌小鼠肿瘤的生长,其可能是通过circ_SLIT3/NF-κB轴调节自噬来实现的。Objective It is to observe the inhibition of decoction for cultivating primordium and resisting cancer on tumor growth in Huh7 hepatocellular carcinoma mice and its effects on circRNA slit guidance ligand 3(circ_SLIT3)/nuclear factor-κB(NF-κB)axis and autophagy of tumor cells.Methods Twelve male C57BL/6 mice were selected to establish models of hepatocellular carcinoma by subcutaneous inoculation of hepatocellular carcinoma Huh7 cell suspension in the right axilla.The successfully modeled mice were randomly divided into model group and group of decoction for cultivating primordium and resisting cancer,and were given normal saline and 2 g/mL decoction for cultivating primordium and resisting cancer by gavage,respectively.After 11 d of continuous intervention,the mice were executed,the tumor volume was calculated according to the length and width of the tumor,the tumor tissue was peeled off and weighed and the tumor inhibition rate was calculated,the formation of autophagic vesicles and autophagic vesicles was observed by transmission electron microscopy,the expressions of microtubule-associated protein 1 light chain 3(LC3),Caspase-3,Bcl-2,Bax in tumor tissues were detected by Western blot method,and the expression of circ_SLIT3 mRNA in tumor tissues was detected by PCR.Results Compared with the model group,the general conditions such as mental status,eating and drinking status,etc.of mice in the group of decoction for cultivating primordium and resisting cancer were better,and the tumor volume and weight of the mice were significantly reduced(all P<0.05),cytolysosome and autophagic vesicles in the tumor cells were significantly increased;the ratio of LC3Ⅱ/LC3Ⅰand the relative protein expressions of Caspase-3,Bax and NF-κB in the tumor tissues were all significantly increased(all P<0.05),the relative expressions of Bcl-2 protein and circ_SLIT3 mRNA were all significantly reduced(all P<0.05).Conclusion Decoction for cultivating primordium and resisting cancer can inhibit tumor growth in Huh7 hepatocellular ca

关 键 词：肝癌 培元抗癌汤 circRNA狭缝引导配体3 自噬 核因子-ΚB 

分 类 号：R-332[医药卫生]