籽用美洲南瓜EMS诱变突变体库的构建及表型分析  

Construction and phenotypic analysis of EMS mutant library of the seed-used pumpkin

作　　者：孙明洋 王萍[1] SUN Mingyang;WANG Ping(College of Horticulture and Plant Protection,Inner Mongolia Agricultural University,Hohhot 010011,China)

机构地区：[1]内蒙古农业大学园艺与植物保护学院,呼和浩特010011

出　　处：《西北植物学报》2024年第8期1283-1294,共12页Acta Botanica Boreali-Occidentalia Sinica

基　　金：内蒙古自治区科技计划项目(2021GG0148);内蒙古自治区自然科学基金项目(2021MS03050);内蒙古自治区直属高校基本科研业务费项目“种业振兴领军人才专项基金”(BR22-11-06);内蒙古农业大学动植物品种选育(培育)专项计划项目(YZGC2017018)。

摘　　要：【目的】构建籽用美洲南瓜突变体库,加快籽用美洲南瓜种质资源创新进程,对南瓜品种选育、品种改良以及遗传基础的拓宽具有重要的意义。【方法】利用1.8%诱变剂甲基磺酸乙酯(EMS)处理籽用美洲南瓜ZHL4种子15 h,然后对M_(1)和M_(2)代群体单株进行表型变异观察,同时对M_(2)群体变异株系ZHL4-33进行显微组织结构观察。【结果】(1)M_(2)群体中共筛选到242个突变植株,45种表型变异,变异类型涵盖了突变株的各个生长时期以及各植物器官,总的突变频率达到25.17%。(2)突变体叶片栅栏组织厚度显著高于野生型,排列紧凑,维管形成层痕迹明显;突变体茎维管束多而密集,导管直径小于野生型,髓部发达,细胞间隙较小,细胞数目有所增加。【结论】初步构建了由425个M_(2)家系所组成的籽用美洲南瓜突变体库,为籽用美洲南瓜功能基因组研究和新品种选育奠定了材料基础。[Objective]This study aims to accelerate the innovation process of germplasm resources of the seed-used pumpkin(Cucurbita pepo L.)by constructing a mutant library,which is critical for variety selection and improvement of the genetic basis.[Methods]1.8%ethyl methanesulfonate(EMS)was used to treat ZHL4 seeds of the seed-used pumpkin for 15 h.Phenotypic variation was observed for M_(1) and M_(2) populations.Microstructural observation was performed for the mutant line ZHL4-33 of the M_(2) population.[Results](1)A total of 242 mutant plants with 45 phenotypic variations were screened in the M_(2) population,and the type of variations covered all growth periods and plant organs of the mutant plants,with a total mutation frequency of 25.17%.(2)Leaf microstructure showed that the thickness of the fenestrated tissue of the mutant was higher than the wild type,and the arrangement was compact and the traces of the vascular layer were obvious.Stem microstructure showed that the vascular bundles of the mutant were more and densely packed,and the conduit diameter was smaller than the wild type.The pith was well developed,with a small cell gap and an increase in cell numbers.[Conclusion]A mutant library of 425 M_(2) lines has been constructed,which lays a foundation for functional genomic analysis of the seed-used pumpkin as well as the selection and breeding of new varieties of seed-used pumpkin.

关 键 词：籽用美洲南瓜 EMS 表型分析 突变体库 显微组织结构 

分 类 号：S642.6[农业科学—蔬菜学]