长链非编码RNA叉头框蛋白D2相邻对链RNA1在胶质瘤细胞中的表达和功能  

The expression and function of LncRNA FOXD2-AS1 in glioma cells

作　　者：毕玉旭 龚鹏程 付愈 姚乾[2] 倪炜[1] BI Yuxu;GONG Pengcheng;FU Yu;YAO Qian;NI Wei(Department of Neurosurgery,the Third Affiliated Hospital of Kunming Medical University,Yunnan Cancer Hospital,Kunming,Yunnan 650000,China;Tumor Research Institute,the Third Affiliated Hospital of Kunming Medical University,Yunnan Cancer Hospital,Kunming,Yunnan 650000,China)

机构地区：[1]昆明医科大学第三附属医院,云南省肿瘤医院神经外科,云南昆明650000 [2]昆明医科大学第三附属医院,云南省肿瘤医院肿瘤研究所,云南昆明650000

出　　处：《中华神经外科疾病研究杂志》2024年第4期7-13,共7页Chinese Journal of Neurosurgical Disease Research

基　　金：昆医联合专项-面上项目(202101AY07001-175)。

摘　　要：目的研究长链非编码RNA叉头框蛋白D2相邻对链RNA1(lncRNA FOXD2-AS1)对胶质瘤生物学行为的影响。方法首先用qPCR实验检测FOXD2-AS1在U251、LN229、U87这3种胶质瘤细胞系中的表达情况,筛选出高表达的2种细胞系进行培养,再构建慢病毒载体转染2种细胞系敲低FOXD2-AS1作为实验组,将空载慢病毒颗粒转染的细胞作为阴性对照组,将常规培养未进行转染的细胞作为空白组,采用划痕实验、Transwell实验检测2种胶质瘤细胞系中各组细胞迁移及侵袭能力,将FOXD2-AS1敲低后进行转录组测序,进行差异基因取交集,并对共同差异基因进行富集分析。结果qPCR实验显示FOXD2-AS1在LN229、U251这2种胶质瘤细胞系中明显高表达,与阴性对照组和空白组相比,被慢病毒载体转染敲低FOXD2-AS1时,2种胶质瘤细胞系中实验组细胞迁移及侵袭能力明显下降。生信数据库分析敲低FOXD2-AS1时发现2种胶质瘤细胞系共有48个相关差异性表达基因,进一步通过KEGG通路富集分析发现,2种胶质瘤细胞系中FOXD2-AS1敲低都与多种信号通路(细胞过程中的聚焦粘附、环境信息处理中的神经活性配体-受体相互作用、遗传信息处理中的剪接体、人类疾病中的癌症通路、新陈代谢中的代谢途径、有机系统中的补体和凝血级联反应)存在相关性。结论LncRNA FOXD2-AS1在胶质瘤细胞迁移、侵袭中发挥作用,其可能的作用机制可还有待后续进一步研究。Objective To study on the impact of lncRNA FOXD2-AS1 on the biological behavior of glioma.Methods Firstly,the expression of FOXD2-AS1 in three glioma cell lines,namely U251,LN229,and U87,was detected by qPCR experiment.Two cell lines with high expression were screened for culturing.Subsequently,lentiviral vectors were constructed to transfect the two cell lines to knockdown FOXD2-AS1 as the experimental group.The cells transfected with empty lentiviral particles were used as the negative control group,and the cells cultured conventionally without transfection were used as the blank group.The scratch test and Transwell test were used to detect the migration and invasion ability of each group of cells in the two glioma cell lines.Transcriptome sequencing was performed after Knockdown of FOXD2-AS1,and the intersection of differential genes was taken,and enrichment analysis was performed on the common differential genes.Results The qPCR experiment showed that FOXD2-AS1 was significantly highly expressed in the two glioma cell lines LN229 and U251.Compared with the negative control group and the blank group,when FOXD2-AS1 was Knockdown by lentiviral vector transfection,the migration and invasion ability of the experimental group cells in the two glioma cell lines decreased significantly.Bioinformatics database analysis found that there were 48 related differentially expressed genes in the two glioma cell lines when FOXD2-AS1 was Knockdown.Further enrichment analysis through KEGG pathway found that the Knockdown of FOXD2-AS1 in the two glioma cell lines was correlated with multiple signaling pathways such as focal adhesion in cell processes,neuroactive ligand-receptor interaction in environmental information processing,spliceosome in genetic information processing,cancer pathways in human diseases,metabolic pathways in metabolism,complement and coagulation cascade in organic systems.Conclusion LncRNA FOXD2-AS1 plays a role in the migration and invasion of glioma cells,and its possible mechanism of action may require fu

关 键 词：长链非编码RNA FOXD2-AS1 胶质瘤 迁移 侵袭 

分 类 号：R739.4[医药卫生—肿瘤]