四川山区63份马铃薯亲本材料的SSR指纹图谱构建及遗传多样性分析  

Construction of SSR Fingerprints and Genetic Diversity Analysis of 63 Potato Materials in Mountainous Regions of Sichuan Province

作　　者：李佩华 张伦[2] 罗杰 杜婷婷 董攀 彭正松[2] 刘登才[1] LI Peihua;ZHANG Lun;LUO Jie;DU Tingting;DONG Pan;PENG Zhengsong;LIU Dengcai(Triticeae Research Institute,Sichuan Agricultural University,Chengdu 611130,China;College of Agricultural Sciences,Xichang University,Xichang 615013,Sichuan,China;School of Life Sciences,Chongqing University,Chongqing 400044,China)

机构地区：[1]四川农业大学小麦研究所,成都611130 [2]西昌学院农业科学学院,四川西昌615013 [3]重庆大学生命科学学院,重庆400044

出　　处：《四川农业大学学报》2024年第4期792-799,共8页Journal of Sichuan Agricultural University

基　　金：“十四五”四川省薯类育种攻关(2021YFYZ0019);国家现代农业产业技术体系四川薯类创新团队(川财教[2019159号])。

摘　　要：【目的】针对近年筛选出的适宜在四川山区种植的63份马铃薯骨干亲本材料开展遗传多样性研究。【方法】从文献中找到208对多态性较高的引物,提取供试材料的DNA进行PCR扩增。【结果】168对引物出现扩增条带,145对引物具有多态性,7对引物可以完全区分供试材料。【结论】用这7对引物构建SSR指纹图谱,发现63份马铃薯材料间的遗传相似系数为0.48~0.98。遗传相似系数为0.481处将供试材料分为A、B和C 3个类群,0.614处将供试材料分为7个类群,表明供试材料间亲缘关系较远,可为马铃薯的遗传育种杂交亲本组配提供参考。【Objective】This experiment aimed to study the genetic diversity of 63 potato backbone parent materials suitable for cultivation in the mountainous regions of Sichuan Province.【Method】We identified 208 primers with high polymorphism from published literature.DNA was extracted from the 63 potato samples and amplified using PCR.【Result】The analysis revealed that 168 primers produced amplified bands,145 primers exhibited polymorphism,and 7 primers were able to completely distinguish the tested materials.【Conclusion】Utilizing these 7 pairs of primers to construct SSR fingerprints,the genetic similarity coefficients among the 63 potato materials ranged from 0.48 to 0.98.At a genetic similarity coeffi-cient of 0.481,the tested materials were categorized into three groups:A,B and C.At a coefficient of 0.614,the materials were further divided into seven groups.This indicates that the tested materials are genetically distant from one another,providing valuable insights for the genetic breeding of potato hybrid parents.

关 键 词：马铃薯 SSR标记 遗传多样性分析 DNA指纹图谱 

分 类 号：S532[农业科学—作物学] S326