液液微萃取−高效液相色谱法测定水中磺胺类药物的实验设计  

Experimental Design for the Determination of Sulfonamide Drugs in Water Using Liquid-Liquid Microextraction Combined with High Performance Liquid Chromatography

作　　者：梁淑彩[1] 廖晓燕[1] 刘毅[1] LIANG Shucai;LIAO Xiaoyan;LIU Yi(School of Pharmaceutical Sciences,Wuhan University,Wuhan 430070,China)

机构地区：[1]武汉大学药学院,武汉430070

出　　处：《实验科学与技术》2024年第4期1-6,共6页Experiment Science and Technology

基　　金：武汉大学本科教育质量建设综合改革项目(20238302);武汉大学专业学位特色课程“药物植物生物技术”建设项目。

摘　　要：基于绿色分析化学理念,设计了液液微萃取-高效液相色谱(HPLC)法测定水中磺胺类药物的实验并应用于本科实践教学。实验中采用较少的萃取溶剂对样品进行前处理,色谱分离时选用乙醇作为绿色有机流动相,通过优化萃取和分离条件,建立了一种液液微萃取-高效液相色谱联用分析水样中磺胺类药物(磺胺胍(SG)、磺胺嘧啶(SD)、磺胺间二甲氧嘧啶(SDM)、磺胺甲噁唑(SMZ)和酞磺胺噻唑(PST))及其常用配伍药甲氧苄啶(TMP)的新方法。其结果表明测定各药物的线性范围分别为10~1000μg/L(SG、SD、SDM及SMZ)、100~1000μg/L(TMP)和50~1000μg/L(PST),检测限在50~100 ng/L(S/N=3)之间。对湖水样品进行测定,加样回收率在93.6%~105.2%之间。该实验立足于分析化学前沿且难易适中,可以作为化学、药学和环境等专业高年级本科生的研究性实验,有利于学生提高创新能力、低碳意识及综合素质。With the concept of green analytical chemistry,an experiment on the determination of sulfonamide drugs in water using liquid-liquid microextraction combined with high-performance liquid chromatography(HPLC)is designed for undergraduate experimental teaching.With a small amount of organic solvents in liquid-liquid extraction and ethanol as the green organic mobile phase for chromatographic separation,the extraction and separation conditions are optimized,and a new method of liquid-liquid microextraction combined with HPLC has been developed for determining sulfonamide drugs(sulfaguanidine(SG),sulfamethoxazole(SD),sulfamethoxazole(SDM),sulfamethoxazole(SMZ)and sulfamethoxazole(PST))and trimethoprim(TMP,a commonly used compatible drug for sulfonamide drugs).The results indicate that the linear measurement ranges are 10~1000μg/L for SG,SD,SDM and SMZ,and 100~1000μg/L for TMP and 50~1000μg/L for PST,respectively.The detection limits are in the ranges of 50~100 ng/L(S/N=3).The recovery rates of the lake water sample are between 93.6%and 105.2%.This experiment is based on the forefront of analytical chemistry and is moderately difficult.It is suitable as a researching experiment for senior undergraduate students majoring in chemistry,pharmacy and environment,which is beneficial for students to improve their innovation ability,low-carbon awareness and comprehensive quality.

关 键 词：液液微萃取 高效液相色谱 磺胺类药物 研究性实验 

分 类 号：O633[理学—高分子化学]