表面等离子共振传感器在生物分子检测中的应用进展  

Advances in the application of surface plasmon resonance sensors for rapid detection of biomolecules

作　　者：胡馨儿 戚敏钰 张颖 曹岩 张海 HU Xiner;QI Minyu;ZHANG Ying;CAO Yan;ZHANG Hai(Shanghai First Maternity and Infant Hospital,School of Medicine,Tongji University,Shanghai 200092,China;School of Pharmacy,Naval Medical University,Shanghai 200433,China)

机构地区：[1]同济大学医学院同济大学附属妇产科医院,上海200092 [2]海军军医大学药学院,上海200433

出　　处：《沈阳药科大学学报》2024年第8期957-965,994,共10页Journal of Shenyang Pharmaceutical University

基　　金：国家自然科学基金资助项目(82174092);上海市自然科学基金资助项目(21ZR1483000);上海市浦江人才计划资助项目(21PJD083)。

摘　　要：生物传感器是一种对生物物质敏感并将可观察的生化反应转变为可测量的物理量的仪器。生物传感器由分子识别元件(抗体、适配体、蛋白质等)、转换元件(氧电极、光敏管等)和信号放大装置组成。待测物质经扩散作用进入分子识别元件,由其识别并发生生物学反应,产生的信息由转换元件转换成可量化和可处理的声、光、电等信号,再经信号放大装置放大并输出,从而得到待测物浓度。生物传感器由传感器检测原理可分为热敏生物传感器、压电生物传感器、光学生物传感器、介体生物传感器等,在食品检测、环境污染、临床诊断、药物发现等方面有广泛的应用。表面离子共振(surface plasmon resonance,SPR)物传感器是一种光学生物传感器,具有无需标记、高选择性、高灵敏度、简易、可靠、低成本、实时响应等优点,广泛应用于抗生素、细菌、病毒、生物毒素、重金属、蛋白、核酸等快速检测。本综述作者对SPR的原理、检测形式和近年来快速检测的应用进行总结,期望能为相关研究提供参考。Objective A biosensor is an instrument that is sensitive to biological substances and converts observable biochemical reactions into measurable physical quantities.Biosensors consist of molecular recognition elements(such as antibodies,aptamers,and proteins),transducers(such as oxygen electrodes and photodiodes),and signal amplification devices.The analyte diffuses into the molecular recognition element,which identifies it and undergoes a biological reaction.The information generated is converted by the transducer into quantifiable and processable signals such as sound,light,or electricity,which are then amplified and output by the signal amplification device,thereby determining the concentration of the analyte.Biosensors can be categorized by their detection principles into types such as thermometric biosensors,piezoelectric biosensors,optical biosensors,and mediator biosensors.They have widespread applications in food testing,environmental pollution monitoring,clinical diagnostics,and drug discovery.Surface plasmon resonance(SPR) sensors are a type of optical biosensor that offer advantages such as label-free detection,high selectivity,high sensitivity,simplicity,reliability,low cost,and real-time response.They are widely used for the rapid detection of antibiotics,bacteria,viruses,biotoxins,heavy metals,proteins,and nucleic acids.This review summarizes the principles,detection methods,and recent applications of SPR in rapid detection,aiming to provide a reference for related research.

关 键 词：表面等离子共振 定量分析 生物医学分析 食品卫生 环境监测 

分 类 号：R917[医药卫生—药物分析学]