氮磷配施对青钱柳生长及叶生物活性物质含量的影响  

Effects of various ratios of nitrogen and phosphorus addition on the growth and contents of leaf bioactive substances in Cyclocarya paliurus

作　　者：刘小芳 岳喜良 方升佐[1,2] 李晴 孙昕[1] LIU Xiaofang;YUE Xiliang;FANG Shengzuo;LI Qing;SUN Xin(College of Forestry and Grassland,Nanjing Forestry University,Nanjing 210037,China;Co-Innovation Center for Sustainable Forestry in Southern China,Nanjing Forestry University,Nanjing 210037,China;Jiangxi Yushan-Yuhechang Agricultural Science and Technology Development Co.,Ltd.,Yushan 334700,China)

机构地区：[1]南京林业大学林草学院,江苏南京210037 [2]南京林业大学,南方现代林业协同创新中心,江苏南京210037 [3]江西玉山县玉合昌农业科技发展有限公司,江西玉山334700

出　　处：《南京林业大学学报（自然科学版）》2024年第4期57-66,共10页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：国家自然科学基金项目(32071750);江苏省重点研发计划(现代农业)重点项目(BE2019388)。

摘　　要：【目的】研究不同水平氮、磷肥配施对青钱柳(Cyclocarya paliurus)生长及叶生物活性物质含量的影响,为青钱柳叶用人工林的定向培育以及提高青钱柳叶生物活性物质含量提供理论依据。【方法】以江西铜鼓种源的青钱柳2年生实生苗为试验材料进行田间试验,采用双因素完全区组试验设计,设置3个施氮水平(N_(0)、N_(1)和N_(2)),3个施磷水平(P_(0)、P_(1)和P_(2)),共9个处理,每个处理3个重复,进行苗木生长指标和叶总黄酮、总三萜、总多酚及总多糖含量测定,采用双因素方差分析比较氮、磷及二者交互作用对相关指标的影响。【结果】氮磷配施对青钱柳幼苗高和地径生长以及叶总黄酮、总三萜、总多酚和总多糖含量均有显著(P<0.05)影响。青钱柳叶总黄酮、总三萜、总多酚含量表现出抛物线形的季节变化,均在7月达最大值,而叶总多糖含量在9月最高。与N_(0)P_(0)处理相比,不同氮磷配施处理均促进了林木生长,且在N_(2)P_(2)处理下树高和地径净生长量最大;但不同氮磷配施处理对叶生物活性物质含量影响不同,且在不同月份以及不同生物活性物质间存在差异。总体看,试验期内各处理间6月叶的总黄酮、总三萜、总多酚和总多糖含量的变化范围分别为17.01~34.25、39.11~55.77、22.74~53.98和22.36~33.24 mg/g,而10月的为16.95~35.26、38.03~54.17、19.78~33.98和23.33~32.46 mg/g。相关分析结果表明,青钱柳苗高月净生长量与对应月的叶生物活性物质含量(总黄酮、总三萜、总多酚和总多糖)相关性不显著(P>0.05),地径月净生长量与对应月的叶次生代谢物含量弱负相关但不显著(P>0.05);青钱柳苗高生长和地径生长之间,以及叶生物活性物质(总黄酮、总三萜、总多酚和总多糖)含量间均存在极显著正相关关系(P<0.01)。【结论】高水平的氮磷肥配施有利于青钱柳生长和叶总三萜含量的提高,中等水平的磷肥有�【Objective】This study aimed to provide a theoretical basis for the strategic cultivation of plantations,and to increase the content of bioactive substances in leaves by investigating the effects of various nitrogen and phosphorus levels on the growth and bioactive substance content in Cyclocarya paliurus leaves.【Method】The research is conducted in the field and utilized a two-factor complete block design,incorporating three levels of nitrogen application(N_(0),N_(1),N_(2))and three levels of phosphorus application(P_(0),P_(1),P_(2)),creating nine treatments with three replicates each.The growth indexes of seedlings and the contents of total flavonoids,total triterpenoids,total polyphenols and total polysaccharides in leaves were determined.A two-way ANOVA was used to compare the effects of nitrogen and phosphorus and their interaction on related indexes.【Result】The study found that different ratios of nitrogen and phosphorus significantly influenced both the growth and the bioactive substance content in C.paliurus leaves(P<0.05).The contents of total flavonoids,triterpenoids,and polyphenols in the leaves exhibited a parabolic seasonal variation,peaking in July,while the highest polysaccharides content was observed in September.Compared to the control treatment(N_(0)P_(0)),all nitrogen and phosphorus fertilizations enhanced tree growth,with the most substantial growth in tree height and ground diameter occurring in the N_(2)P_(2)treatment.The effects of nitrogen and phosphorus ratios on the bioactive substances varied,displaying differences among the substances and the sampling months sampled.The variation in the contents of total flavonoid,total triterpenoids,total polyphenols and total polysaccharides in June ranged from 17.01 to 34.25 mg/g,from 39.11 to 55.77 mg/g,from 22.74 to 53.98 mg/g,and from 22.36 to 33.24 mg/g,respectively,while in October,the ranges were 16.95-35.26,38.03-54.17,19.78-33.98 and 23.33-32.46 mg/g,respectively.Correlation analysis revealed no significant relationship between the

关 键 词：青钱柳 配方施肥 林木净生长量 生物活性物质 

分 类 号：S725[农业科学—林木遗传育种]