基于非靶向代谢组学分析黄色和白色金针菇代谢物差异  

Analysis of metabolite difference between yellow and white strains of Flammulina filiformis based on untargeted metabolomics

作　　者：刘询 何晓兰 贾定洪 彭卫红 王波 LIU Xun;HE Xiaolan;JIA Dinghong;PENG Weihong;WANG Bo(National Observing and Experimental Station of Agricultural Microbiology in Xindu,National-Local Joint Engineering Laboratory of Breeding and Cultivation of Edible and Medicinal Fungi,Sichuan Institute of Edible Fungi,Chengdu 610066,Sichuan,China)

机构地区：[1]四川省食用菌研究所,国家农业微生物新都观测实验站,食药用菌育种与栽培国家地方联合工程实验室,四川成都610066

出　　处：《菌物学报》2024年第8期70-82,共13页Mycosystema

基　　金：国家食用菌产业技术体系(CARS-20)。

摘　　要：金针菇Flammulina filiformis是我国重要的食用真菌,主要包括黄色和白色两种品系。为了探究不同颜色金针菇子实体代谢物的差异,以相同条件下栽培的黄色和白色金针菇子实体为供试材料进行非靶向代谢组学分析。结果显示:大部分差异代谢物在黄色金针菇中的积累量明显较高,黄色和白色子实体之间的差异代谢物显著富集到苯丙氨酸和酪氨酸及色氨酸生物合成途径、淀粉和蔗糖代谢途径。黄色金针菇中苯丙氨酸含量降低同时酪氨酸含量增加,对应的苯丙氨酸解氨酶基因PAL1的表达水平下调,酪氨酸酶基因TYR表达上调;苯丙氨酸解氨酶和酪氨酸酶的活性均显著高于白色金针菇。此外,益智或呈味相关氨基酸半胱氨酸、精氨酸、脯氨酸、天冬氨酸和组氨酸在黄色金针菇中含量更高。本研究整体呈现了两种金针菇的代谢物差异并为后续完整解析子实体颜色表型差异提供了线索和铺垫。Flammulina filiformis is an important edible fungus in China,which mainly includes yellow and white strains.For the purpose of understanding their metabolite variations,yellow and white strains of F.filiformis grown under identical conditions were chosen for untargeted metabolomics analysis.The results showed that most of the differentially accumulated metabolites(DAMs)were significantly higher in the yellow strain,and the metabolite difference between fruiting bodies of yellow and white strains were significantly enriched to phenylalanine and tyrosine as well as tryptophan biosynthesis pathway,and starch and sucrose metabolism pathway.The content of phenylalanine decreased and that of tyrosine increased in yellow strain;correspondingly the expression level of phenylalanine ammonia-lyase gene PAL1 was down-regulated,and that of tyrosinase gene TYR up-regulated.The activity of phenylalanine ammonia-lyase and tyrosinase in fruiting body of yellow strain was significantly higher than that in fruiting body of white strain.The content of cysteine,arginine,proline,aspartic acid and histidine was higher in yellow strain than that in white strain.This study provides a clue to subsequent complete analysis of color phenotypic differences of the two strains.

关 键 词：金针菇 颜色 代谢组学 代谢物 

分 类 号：S646.15[农业科学—蔬菜学]