1株甲周脓肿患者分离的雷金斯堡预研菌的鉴定及生物学特征分析  

Identification and characterization of one Yokenella regensburgei isolated from a case of perionychial abscess

作　　者：李振起 刘亚东 赵海飞 袁帆 张雪英[5] 王雅轩 程欣 Li Zhenqi;Liu Yadong;Zhao Haifei;Yuan Fan;Zhang Xueying;Wang Yaxuan;Cheng Xin(Department of Clinical Laboratory,Shan County Central Hospital,Heze 274300,China;State Key Laboratory of Microbial Resources,Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China;Department of Imaging,Huaihai Hospital Affiliated to Changzhi Medical College,Changzhi 046000,China;Department of Otolaryngology,Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China;Department of Dermatology,Shan County Central Hospital,Heze 274300,China;Beijing Hospital/National Center of Gerontology,Center for Clinical Laboratories,National Health Commission of the PRC,Institute of Geriatric Medicine,Chinese Academy of Medical Sciences,Beijing 100730,China;Department of Clinical Laboratory,Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China)

机构地区：[1]单县中心医院检验科,菏泽274300 [2]中国科学院微生物研究所,微生物资源前期开发国家重点实验室,北京100101 [3]长治医学院附属淮海医院影像科,长治046000 [4]首都医科大学附属北京友谊医院耳鼻喉科,北京100050 [5]单县中心医院皮肤科,菏泽274300 [6]北京医院国家老年医学中心,国家卫生健康委员会临床检验中心,中国医学科学院老年医学研究院,北京100730 [7]首都医科大学附属北京友谊医院临床检验中心,北京100050

出　　处：《中华微生物学和免疫学杂志》2024年第8期689-695,共7页Chinese Journal of Microbiology and Immunology

摘　　要：目的对1株甲周脓肿患者分离的少见病原体雷金斯堡预研菌进行菌种鉴定和生物学特性分析。方法菌株CXLZQ123于2023年6月2日分离自单县中心医院皮肤科门诊就诊的甲周脓肿患者,经过初步形态学鉴定、生化鉴定,后经质谱鉴定,16S rRNA测序和全基因组测序;应用MEGA 11.0对其与GenBank中相近种属进行种群间比对,并基于遗传距离构建系统发育树分析其遗传学进化情况。同时比较与类似菌株基因组平均核苷酸一致性。结果该菌株为革兰阴性杆菌,MicroScan WalkAway生化鉴定该菌为雷金斯堡预研菌(91.47%)或蜂房哈夫尼菌(8.53%)。梅里埃质谱鉴定为雷金斯堡预研菌。经16S rRNA基因序列分析该菌株与CIP 105435(序列号NR104934.1)相似性最高(相似度99.37%)。将该分离菌株雷金斯堡预研菌16S rRNA基因序列上传至美国国家生物技术信息中心(NCBI,GenBank序列号:OR230248.1)。基因组测序上传序列(NCBI,SRA序列号:SRR26510420)。该菌株与雷金斯堡预研菌W13和UU2206353平均核苷酸一致性分别为98.82%和99.04%。结论该致病菌通过形态学观察、生化鉴定、质谱鉴定、16S rRNA和全基因组测序鉴定为雷金斯堡预研菌。该菌比较少见,对大多数药物敏感。本研究可为雷金斯堡预研菌相关感染提供诊治经验。ObjectiveTo identify and characterize one Yokenella regensburgei strain(designated as CXLZQ123)isolated from a case of perionychial abscess.MethodsStrain CXLZQ123 was isolated from a patient with periungual abscess at the Dermatology Department of San County Central Hospital in June 2,2023.The strain was initially identified through morphological and biochemical tests,followed by mass spectrometry identification,16S rRNA sequencing and whole-genome sequencing.MEGA 11.0 was used to compare and analyze the strain′s genetic relationship with relevant species in GenBank,and a phylogenetic tree was constructed based on genetic distance to analyze its genetic evolution.Meanwhile,the average nucleotide identity between its genome and similar strains were compared.ResultsThe strain was identified as a Gram-negative rod.MicroScan WalkAway biochemical tests indicated that the strain was either Yokenella regensburgei(91.47%)or Hafnia alvei(8.53%).MALDI-TOF mass spectrometry confirmed it as Yokenella regensburgei.Based on 16S rRNA gene sequence analysis,the strain showed the highest similarity(99.37%)to CIP 105435(sequence number NR_104934.1).The 16S rRNA gene sequence of the isolated strain Yokenella regensburgei was submitted to the National Center for Biotechnology Information(NCBI)with the GenBank sequence number of OR230248.1.The whole-genome of CXLZQ123 were sequenced and uploaded(NCBI,SRA sequence number:SRR26510420).The average nucleotide identity between CXLZQ123 and Yokenella regensburgei strains W13 and UU2206353 were 98.82% and 99.04%,respectively.ConclusionsThrough morphological observation,biochemical identification,mass spectrometry identification,16S rRNA and whole-genome sequencing,this pathogenic strain is identified as Yokenella regensburgei.This rare bacterium is sensitive to most detected antibiotics.This study provides diagnostic and treatment experience for Yokenella regensburgei-related infections.

关 键 词：雷金斯堡预研菌 鉴定 16S rRNA 系统发育树 全基因组测序 

分 类 号：R446.5[医药卫生—诊断学]