‘壶瓶枣’果皮着色物质及其相关基因筛选  

Screening of Ziziphus jujuba‘Hupingzao’Fruit Coloring Substances and Their Related Genes

作　　者：李洁[1] 武超 贾祥堑 王娟[1] LI Jie;WU Chao;JIA Xiangqian;and WANG Juan(College of Forestry Shanxi Agricultural University,Taigu,Shanxi 030801,China)

机构地区：[1]山西农业大学林学院,山西太谷030801

出　　处：《园艺学报》2024年第8期1728-1742,共15页Acta Horticulturae Sinica

基　　金：山西农业大学博士科研启动项目(2020BQ28)。

摘　　要：以‘壶瓶枣’(Ziziphusjujuba‘Hupingzao’)白熟期、半红期、全红期果实为试材,测定果皮主要色素物质含量并进行转录组测序,从分子水平阐述果皮着色基因的变化。结果表明,白熟期后,果皮花色苷含量开始显著降低;3个时期果皮类黄酮含量均依次显著降低;叶绿素含量在白熟期到半红期变化不大,全红期显著下降;类胡萝卜素含量在转色阶段相对稳定。类黄酮、花色苷、类胡萝卜素和叶绿素是‘壶瓶枣’果皮的着色物质,且花色苷分为飞燕草素、矢车菊素、矮牵牛素、锦葵色素、芍药色素和天竺葵色素等6种。根据转录组测序中GO和KEGG富集分析发现,在类黄酮、花色苷、黄酮与黄酮醇、类胡萝卜素和卟啉代谢通路中差异基因个数分别为35、22、6、30和38。其中,白花青素还原酶基因(LOC107429483)、转录因子基因MYC2(LOC107430586)和二氢黄酮醇4–还原酶基因(LOC107420289)在果皮着色中发挥主要作用,β–胡萝卜素羟化酶基因(LOC107414761)是影响类胡萝卜素合成的关键基因;卟啉代谢中叶绿体A加氧酶基因(LOC107410684)是影响总叶绿素含量的关键基因。The white ripening,semi-red and whole red fruits of Ziziphus jujuba‘Hupingzao’as materials,the contents of the main pigment substances in the peel were determined and transcriptome sequencing was carried out to elaborate the changes of coloring genes in the peel of jujube at the molecular level.The results showed that after the white ripening period,the anthocyanin content of jujube peel began to decrease significantly.In the three periods,the flavonoid content of jujube peel decreased significantly.The chlorophyll content did not change much from the white maturity stage to the semi-red stage,and decreased significantly at the full red stage.The content of carotenoids was relatively stable in the veraison stage.Flavonoids,anthocyanins,carotenoids and chlorophyll are the coloring substances of jujube peel,and the anthocyanins are divided into six types:delphinidin,cyanidin,petunidin,malvidin,peonidin and pelargonidin.According to the GO and KEGG enrichment analysis in transcriptome sequencing,the number of differential genes in flavonoids,anthocyanins,flavonoids and flavonols,carotenoids and porphyrin metabolic pathways were 35,22,6,30 and 38,respectively.Among them,the white anthocyanidin reductase gene(LOC107429483),transcription factor gene MYC2(LOC107430586)and dihydroflavonol 4-reductase gene(LOC107420289)played a major role in the coloring of jujube peel,andβ-carotene hydroxylase gene(LOC107414761)was the key gene affecting carotenoid synthesis.The chloroplast A oxygenase gene(LOC107410684)in porphyrin metabolism is a key gene affecting total chlorophyll content.

关 键 词：枣 着色 转录组 类黄酮 花色苷 

分 类 号：S665.1[农业科学—果树学]