应用低频波动振幅技术评估多菌种益生菌对失眠障碍患者脑功能的影响  

Amplitude of low-frequency fluctuation in evaluating the effect of multi-strain probiotics on brain function of insomnia disorder patients

作　　者：闫峰山 张淼 谷宇昂 贾淑蕾 武肖玲 邹智 李中林 王升辉 周菁 尚菲菲 李永丽 YAN Fengshan;ZHANG Miao;GU Yuang;JIA Shulei;WU Xiaoling;ZOU Zhi;LI Zhonglin;WANG Shenghui;ZHOU Jing;SHANG Feifei;LI Yongli(Department of Medical Imaging,Henan Provincial People's Hospital,Zhengzhou University People's Hospital,Zhengzhou,Henan 450003,China;Grade 2022,Xinriang Medical University,Xinriang,Henan 453003,China;Department of Nuclear Medicine,Henan Provincial People's Hospital,Zhengzhou University People's Hospital,Zhengzhou,Henan 450003,China;Department of Neurology,Henan Provincial People's Hospital,Zhengzhou University People's Hospital,Zhengzhou,Henan 450003,China;Department of Health Management,Henan Provincial People's Hospital,Zhengzhou University People's Hospital,Zhengzhou,Henan 450o03,China)

机构地区：[1]河南省人民医院医学影像科,郑州大学人民医院,河南郑州450003 [2]新乡医学院,河南新乡453003 [3]河南省人民医院核医学科,郑州大学人民医院,河南郑州450003 [4]河南省人民医院神经内科,郑州大学人民医院,河南郑州450003 [5]河南省人民医院健康管理学科,郑州大学人民医院,河南郑州450003

出　　处：《中华实用诊断与治疗杂志》2024年第8期757-761,共5页Journal of Chinese Practical Diagnosis and Therapy

基　　金：国家自然科学基金(82071884);中原科技创新领军人才计划(244200510016);河南省医学科技攻关计划项目(SBGJ202302011);河南省科技攻关计划项目(242102311018)。

摘　　要：目的应用静息态功能磁共振成像(rs-fMRI)-低频波动振幅(ALFF)技术分析失眠障碍(ID)患者应用益生菌干预前后局部脑区神经活动的强度变化,探讨益生菌对ID患者睡眠、情绪的改善作用。方法2022年12月—2023年12月河南省人民医院诊治ID患者32例,随机分为观察组20例和对照组12例。观察组给予多菌种益生菌(由瑞士乳杆菌、副干酪乳杆菌、嗜热链球菌组成),对照组给予包装及规格与多菌种益生菌相同的安慰剂,均每日晚饭后温水冲服或直接口服,6 g/d,连续4周。比较2组干预前、干预4周后匹兹堡睡眠质量指数(PSQI)、失眠严重程度指数(ISI)、汉密尔顿焦虑量表(HAMA)、汉密尔顿抑郁量表(HAMD)评分;采集2组干预前、干预4周后rs-fMRI数据进行ALFF分析,计算ALFF值。结果干预前观察组PSQI[(14.400±2.872)分]、ISI[(15.950±5.083)分]、HAMA[(10.200±8.575)分]、HAMD[(12.150±7.842分)]评分与对照组[(13.250±2.340)、(13.917±4.316)、(14.167±9.331)、(11.833±4.529)分]比较差异均无统计学意义(t=0.492,P=0.488;t=1.484,P=0.233;t=0.746,P=0.359;t=1.856,P=0.183)。观察组干预后PSQI[(9.200±2.984)分]、ISI[(10.750±5.693)分]、HAMA[(9.465±7.300)分]、HAMD[(8.350±7.205)分]评分均低于干预前(t=7.984,P<0.001;t=4.690,P=0.001;t=2.610,P=0.017;t=2.916,P=0.009);对照组干预后HAMA评分[(11.167±7.481)分]低于干预前(t=2.324,P=0.040),PSQI[(12.667±2.535)分]、ISI[(13.250±5.561)分]、HAMD[(12.250±5.011)分]评分与干预前比较差异均无统计学意义(t=1.541,P=0.152;t=0.842,P=0.417;t=-0.392,P=0.702)。干预后观察组PSQI评分低于对照组(t=-3.358,P=0.002),ISI、HAMA、HAMD评分与对照组比较差异均无统计学意义(t=-1.213,P=0.235;t=-1.205,P=0.238;t=-1.646,P=0.110)。观察组干预后右侧颞上回ALFF值低于干预前(GRF校正,体素水平P<0.01,团块水平P<0.05);对照组未发现干预前后ALFF值差异有统计学意义的脑区。结论ID患者应用多菌种益生菌后睡眠�Objective To analyze the changes in the intensity of neural activity in local brain regions of insomnia disorder(ID)patients by resting-state functional magnetic resonance imaging(rs-fMRI)-amplitude of low-frequency fluctuation(ALFF)technique before and after probiotic intervention,and to invesitage the effect of probiotics on improving sleep and mood of ID patients.Methods Thirty-two ID patients were diagnosed and treated in Henan Provincial People's Hospital from December 2022 to December 2023,and were randomly divided into observation group(n=20)and control group(n=12).The patients in observation group received multi-strain probiotics(composed of Lactobacillus Swiss,Lactobacillus paracaseus and Streptococcus thermophilus),and the patients in control group took 6 g/d placebo which had the same packaging and specifications as the multi-strain probiotics,in warm water or directly swalloned after dinner,for 4 consecutive weeks.The Pittsburgh Sleep Quality Index(PSQI),Insomnia Severity Index(ISI),Hamilton Anxiety Scale(HAMA),and Hamilton Depression Scale(HAMD)scores were compared between two groups before and 4 weeks after intervention.The rs-fMRI data were collected for ALFF analysis before and 4 weeks after intervention,and the ALFF value was calculated.Results The scores of PSQI,ISI,HAMA and HAMD showed no significant differences between observation group(14.400±2.872,15.950±5.083,10.200±8.575,12.150±7.842)and control group(13.250±2.340,13.917±4.316,14.167±9.331,11.833±4.529)before intervention(t=0.492,P=0.488;t=1.484,P=0.233;t=0.746,P=0.359,t=1.856,P=0.183),and were lower in observation group after intervention(9.200±2.984,10.750±5.693,9.465±7.300,8.350±7.205)than those before intervention(t=7.984,P<0.001;t=4.690,P=0.001;t=2.610,P=0.017;t=2.916,P=0.009).In control group,the HAMA score was lower after intervention(11.167±7.481)than that before intervention(t=2.324,P=0.040),and the scores of PSQI,ISI and HAMD were not significant different after intervention(12.667±2.535,13.250±5.561,12.250±5.011)

关 键 词：失眠障碍 益生菌 静息态功能磁共振成像 低频波动振幅 

分 类 号：R740[医药卫生—神经病学与精神病学]