基于肠道菌群与相关激素因子的交互作用探讨麦芽水煎液对缺乳症模型大鼠催乳作用  

Exploration of the lactogenic effect of Hordei Fructus Germinatus water decoction on hypogalactia model rats based on the interaction between intestinal flora and related hormone factors

作　　者：白浩东 张子东 孙延平[1] 祝明涛 张志宏[1] 王秋红 匡海学[1] BAI Hao-dong;ZHANG Zi-dong;SUN Yan-ping;ZHU Ming-tao;ZHANG Zhi-hong;WANG Qiu-hong;KUANG Hai-xue(Key Laboratory of Chinese Materia Medica under Ministry of Education/Heilongjiang Key Laboratory of Traditional Chinese Medicine Pharmacodynamic Material Bases,Heilongjiang University of Chinese Medicine,Harbin 150040,China;School of Life Science,Guangzhou University,Guangzhou 510006,China;School of Chinese Materia Medica,Guangdong Pharmaceutical University,Guangzhou 510006,China)

机构地区：[1]黑龙江中医药大学教育部北药基础与应用研究重点实验室/黑龙江省中药及天然药物药效物质基础研究重点实验室,哈尔滨150040 [2]广州大学生命科学学院,广州510006 [3]广东药科大学中药学院,广州510006

出　　处：《中国新药杂志》2024年第16期1708-1718,共11页Chinese Journal of New Drugs

基　　金：国家中医药管理局中医药传承与创新“百千万”人才工程-岐黄工程首席科学家支持项目(国中医药人教函[2021]7号);2022年全国名老中医药专家传承工作室建设项目(国中医药人教函[2022]75号);2023年度基础与应用基础研究专题项目(SL2022A04J01286)。

摘　　要：目的:探究麦芽水煎液对缺乳症模型大鼠体重、催乳水平、激素水平、乳腺组织以及肠道菌群的影响。方法:通过调整每窝仔鼠的数量建立缺乳症大鼠模型,根据体重随机分为5组:模型组、对照组和麦芽水煎液低、中、高剂量组,每组10只。模型组大鼠生理盐水灌胃,对照组甲氧氯普胺片(1.870 mg·kg^(-1)·d^(-1))灌胃,麦芽水煎液组低、中、高剂量(0.900,1.800,3.600 mg·kg^(-1)·d^(-1))麦芽水煎液灌胃,干预15 d,测定各组大鼠每小时泌乳量、体重、仔鼠体重净增加量和血清中催乳素(prolactin,PRL)、雌二醇(estradiol,E_(2))、生长激素(growth hormone,GH)水平,检测乳腺组织病理学改变和肠道菌群改变。采用Pearson相关性分析法,考察大鼠血清中相关激素因子与体内肠道菌群相关性。结果:干预15 d后,麦芽水煎液中剂量组可显著提高大鼠体重,促进每小时泌乳量,提高仔鼠体重,大鼠血清中PRL,E_(2)和GH水平升高(P<0.05);病理学检测发现,麦芽水煎液中剂量组与模型组相比,腺泡数目明显增多,腺腔高度充盈,小叶间脂肪与纤维结缔组织明显减少,各个腺腔内均可见大量粉色分泌物;肠道菌群分析显示,麦芽水煎液组与模型组相比,拟杆菌门、厚壁菌门丰度提高,疣微菌门、变形菌门丰度下降。建立机体激素因子与肠道菌群相关性分析发现,拟杆菌属嗜果胶拟杆菌(Bacteroides_pectinophilus_group)与PRL,E_(2)呈负相关(P<0.05),与GH呈显著负相关(P<0.01);多尔氏菌属(Dorea)与E_(2)呈正相关(P<0.05);弧菌属(Vibrio)与E_(2)呈负相关(P<0.05)。Tax4Fun功能预测结果表明麦芽水煎液主要与类固醇降解、多巴胺能突触信号传导等通路有关。结论:麦芽水煎液对于缺乳症模型大鼠的泌乳水平有一定的改善作用,可调节大鼠肠道菌群结构,改善相关激素因子分泌水平,差异菌种与3个激素因子呈相关性,提示可通过调节激素分泌的相关因子与菌群组�Objective:To investigate the effects of Hordei Fructus Germinatus(HFG)water decoction on body weight,prolactin level,hormone level,mammary gland tissue,and intestinal microflora in agalactia model rats.Methods:The agalactia model was established by adjusting the number of rats in each litter.SD rats were randomly divided into five groups according to their body weight:model group,control group,and low,medium,and high-dose HFG decoction group,with 10 rats in each group.The model group was gavaged with normal saline,the control group was gavaged with metoclopramide(1.870 mg·kg^(-1)),and the HFG group was given low,medium and high doses(0.900,1.800,3.600 mg·kg^(-1))for 15 d.The hourly milk yield,body weight,pups weight gain of rats were determined in each group.The serum levels of prolactin(PRL),estradiol(E_(2)),growth hormone(GH),breast histopathological,and intestinal microflora changes were measured.The relationship between the intestinal flora in vivo and the pertinent hormone variables in rat serum was examined using Pearson correlation analysis.Results:After 15 days of intervention,the medium-dose group significantly increased the body weights of mother rats and pups rats,milk yield per hour,and the serum levels of PRL,E_(2),and GH(P<0.05).Pathological examination showed that compared with the model group,the number of acini in the medium-dose group was significantly increased,the glandular lumen was highly filled,the interlobular fat and fibrous connective tissue were significantly reduced,and a large quantity of pink secretion was seen in each glandular lumen.According to the examination of intestinal flora,there were more Firmicutes and Bacteroidetes in the HFG water decoction than in the model group,although there were fewer Verrucomicrobia and Proteobacteria.Bacteroides_pectinophilus_group was found to be negatively correlated with PRL and E_(2)(P<0.05)and significantly negatively correlated with GH(P<0.01);the genus Dorea was found to be positively correlated with E_(2)(P<0.05);and the genus Vibrio was

关 键 词：麦芽 缺乳症 乳腺 催乳素 肠道菌群 

分 类 号：R965[医药卫生—药理学]