食源性病原微生物核酸检测技术在铁路食品安全保障中的应用研究  

The Application Prospects of Nucleic Acid-based Techniques for Detection ofFood-borne Pathogen in Railway Food Safety Guarantee

作　　者：靖博彬 邱永祥 王义峰 江华洲 王世明 胡光亚 JING Bobin;QIU Yongxiang;WANG Yifeng;JIANG Huazhou;WANG Shiming;HU Guangya(Energy Saving&Environmental Protection&Occupational Safety and Health Research Institute,China Academy of Railway Sciences Co.,Ltd.,Beijing 100081,China;Nanchang Disease Prevention and Control Institute,China Railway Nanchang Bureau Group Co.,Ltd.,Nanchang Jiangxi 330003,China)

机构地区：[1]中国铁道科学研究院集团有限公司节能环保劳卫研究所,北京100081 [2]中国铁路南昌局集团有限公司南昌疾病预防控制所,江西南昌330003

出　　处：《铁路节能环保与安全卫生》2024年第4期41-47,共7页Railway Energy Saving & Environmental Protection & Occupational Safety and Health

基　　金：中国铁道科学研究院集团有限公司科研开发基金项目(2022YJ211)。

摘　　要：食源性病原微生物已经成为全球性人类疾病和死亡的严重威胁之一,其检测对食品安全至关重要。传统方法由于耗时费力,需要研究应用更为快速、高效、灵敏、经济的检测新技术。在梳理以核酸和各种聚合酶链式反应为基础的食源性病原微生物最新检测技术的基础上,分析各项技术的原理、优点、局限性,探讨在铁路食品安全保障中的应用前景。Food-borne pathogens have become one of the severe threats to human illness and death world-wide.Detection of pathogenic microbes is thus essential for food safety.The conventional methods are time-consuming and laborious,and require research and application of advanced detection techniques that are quick,efficient,highly sensitive and economical.Recent nucleic acid-based and different polymerase chain reaction-based techniques that can be used for detection of viral and bacterial food-borne pathogens in food control laboratories are reviewed herein.Principles,superiority,limitations and application prospects in railway food safety guarantee of these methods are discussed.

关 键 词：食品 病原微生物 核酸检测技术 聚合酶链式反应 

分 类 号：R193[医药卫生—卫生事业管理]