乳腺癌组织中TIMP-3及DNMT1的表达与患者预后的相关性  

The correlation between the expression of TIMP-3 & DNMT1 and prognosis of breast cancer patients

作　　者：于娇[1] 李汉杰[2] 陈鑫[2] 王青[1] 葛鹏[2] 李刚 YU Jiao;LI Hanjie;CHEN Xin;WANG Qing;GE Peng;LI Gang(Department of Radiotherapy,Shaanxi Provincial People's Hospital,Shaanxi Xi'an 710068,China;Department of Thoracic Surgery,the Second Affiliated Hospital of Xi'an Medical University,Shaanxi Xi'an 710038,China)

机构地区：[1]陕西省人民医院放疗科,陕西西安710068 [2]西安医学院第二附属医院胸外科,陕西西安710038

出　　处：《现代肿瘤医学》2024年第17期3248-3253,共6页Journal of Modern Oncology

基　　金：陕西省重点研发计划(编号:2023-YBSF-329);陕西省人民医院科技发展孵化基金(编号:2022YJY-01);陕西省人民医院科技人才支持计划菁英人才项目(编号:2022JY-08);西安医学院第二附属医院博士基金(编号:X2Y-R9)。

摘　　要：目的:分析研究乳腺癌患者中基质金属蛋白酶组织抑制因子3(TIMP-3)及DNA甲基转移酶1(DNMT1)的表达水平与患者临床预后的相关性。方法:收集58例临床资料完整的乳腺癌手术切除标本,采用免疫组化SP法检测癌组织及相应癌旁组织中TIMP-3、DNMT1、ER、PR、HER-2、p53、Ki-67的表达,将TIMP-3、DNMT1表达水平与临床病理参数及随访生存状况进行相关性分析,所有入组患者均进行随访5年以上。结果:我们发现,在乳腺癌组织中,TIMP-3呈低表达,DNMT1呈高表达,TIMP-3及DNMT1的表达呈负相关;TIMP-3表达水平与Ki-67呈负相关,DNMT1表达水平与Ki-67呈正相关,与其他临床病理参数未见相关性。Cox风险比例模型分析显示只有临床分期和DNMT1表达水平是影响总生存期(OS)和无病生存期(DFS)的独立风险因素。TIMP-3低表达组的5年OS和DFS均显著低于高表达组,DNMT1高表达组的5年OS和DFS均显著低于低表达组。结论:研究表明乳腺癌中TIMP-3及DNMT1表达水平与肿瘤细胞恶性表型及患者生存时间有关,可能成为判断乳腺癌预后的一项重要指标,并作为治疗计划制定的依据。Objective:To study the relationship between the expression of tissue inhibitor of matrix metalloproteinase 3(TIMP-3) and DNA methyltransferase 1(DNMT1) and clinical prognosis in breast cancer patients.Methods:58 surgical specimens of breast cancer with complete clinical data were collected,and the expressions of TIMP-3,DNMT1,ER,PR,HER-2,p53,Ki-67 in cancer tissues and corresponding adjacent tissues were detected by immunohistochemical SP method.The expression levels of TIMP-3 and DNMT1 were correlated with clinicopathological parameters and follow-up survival.All patients were followed up for more than 5 years.Results:We found that in breast cancer tissue,TIMP-3 was low expression,DNMT1 was high expression,and the expression of TIMP-3 and DNMT1 was negatively correlated.The expression level of TIMP-3 was negatively correlated with Ki-67,while the expression level of DNMT1 was positively correlated with Ki-67.There was no correlation with other clinical pathological parameters.Cox risk scale model analysis showed that only clinical stage and DNMT1 expression level were independent risk factors affecting overall survival(OS) and disease free survival(DFS).The 5-year OS and DFS of TIMP-3 with low expression were significantly lower than those of the high expression group,while the 5-year OS and DFS of the DNMT1 high expression group were significantly lower than those of the low expression group.Conclusion:This study shows that the expression levels of TIMP-3 and DNMT1 in breast cancer are related to the malignant phenotype of tumor cells and the survival time of patients,which may become an important indicator to judge the prognosis of breast cancer and serve as a basis for the formulation of treatment plans.

关 键 词：乳腺癌 基质金属蛋白酶组织抑制因子3 DNA甲基转移酶1 总生存期 无病生存期 

分 类 号：R737.9[医药卫生—肿瘤]