植物乳杆菌LP-37和氟苯尼考对许氏平鲉(Sebastes schlegelii)肠道形态、消化酶及免疫酶活性的影响  

Effects of Lactobacillus plantarum LP-37 and Florfenicol on Intestinal Histomorphology,and Activities of Digestive Enzymes and Immune Enzymes of Schlegel’s Black Rockfish Sebastes schlegelii

作　　者：宋德青 于晓清 季相山[2] 李莉 王友红 王晓璐 许拉 盖春蕾 樊英 叶海斌 SONG Deqing;YU Xiaoqing;JI Xiangshan;LI Li;WANG Youhong;WANG Xiaolu;XU La;GAI Chunlei;FAN Ying;YE Haibin(Municipal Engineering Research Center of Aquatic Biological Quality Evaluation and Application,Shandong Key Laboratory of Disease Control in Mariculture,Marine Science Institute of Shandong Province,Qingdao 266104,China;Shandong Agricultural University,Tai′an 271018,China)

机构地区：[1]山东省海洋科学研究院(青岛国家海洋科学研究中心),山东省海水养殖病害防治重点实验室,青岛市水产生物品质评价与利用工程研究中心,山东青岛266104 [2]山东农业大学,山东泰安271018

出　　处：《水产学杂志》2024年第4期49-55,共7页Chinese Journal of Fisheries

基　　金：山东省鱼类产业技术体系(SDAIT-12-01);海洋科技创新专项-2023年海洋科技创新示范工程项目(23-1-3-hysf-2-hy);山东省重点研发计划科技示范工程(2021SFGC0701).

摘　　要：为分析植物乳杆菌(Lactobacillus plantarum)LP-37和氟苯尼考对许氏平鲉(Sebastes schlegelii)肠道形态的影响,在水温(14±1)℃条件下,将体质量为(32.23±9.38)g的许氏平鲉饲养在约5 m^(3)的循环水圆桶中45 d,分别投喂4种饲料:喷洒去离子水的基础饲料(对照组C0);前30 d投喂喷洒1×10^(7)CFU/g植物乳杆菌的饲料,后15 d投喂对照饲料(植物乳杆菌组,P1);前30 d投喂喷洒20 mg/L氟苯尼考的饲料,后15 d投喂对照饲料(氟苯尼考组,F1);前15 d投喂喷洒氟苯尼考的饲料,中间15 d投喂喷洒植物乳杆菌的饲料,最后15 d投喂对照饲料(植物乳杆菌和氟苯尼考组,G1)。在第30 d和第45 d解剖许氏平鲉的肠道,通过HE染色和Image J软件分析肠道形态结构的变化,并测定血清中α-淀粉酶、脂肪酶、胰蛋白酶、碱性磷酸酶和超氧化物歧化酶活性的变化。结果显示,1×10^(7)CFU/g植物乳杆菌和20 mg/L的氟苯尼考可以改善肠道结构,显著提高肠道绒毛长度、肌层厚度、隐窝深度(P<0.05);1×10^(7)CFU/g植物乳杆菌LP-37可以显著提高许氏平鲉血清中α-淀粉酶、碱性磷酸酶、超氧化物歧化酶活性(P<0.05);20 mg/L的氟苯尼考可以显著提高许氏平鲉血清α-淀粉酶、胰蛋白酶活性、碱性磷酸酶、超氧化物歧化酶的活性,显著降低脂肪酶活性(P<0.05);组合使用1×10^(7)CFU/g植物乳杆菌LP-37和20 mg/L氟苯尼考可以显著提高许氏平鲉血清α-淀粉酶、超氧化物歧化酶的活性(P<0.05),显著降低脂肪酶、胰蛋白酶的活性(P<0.05)。综上所述,植物乳杆菌LP-37和氟苯尼考均可以改善许氏平鲉肠道结构,调节许氏平鲉消化酶和免疫酶的活性。In order to analyze the effects of Lactobacillus plantarum LP-37 and florfenicol on the intestinal histomorphology of Sebastes schlegelii,with body weight of(32.23±9.38)g were reared in a circulating water tank of about 5 m^(3)for 45 d at water temperature of(14±1)℃,and fed four diets were fed separately:basal diet sprayed with deionized water(control group,C0);die with 1×10^(7)CFU/g L.plantarum was sprayed for 30 d,followed by control diet(P1)for 15 d;diet sprayed with 20 mg/L florfenicol for 30 d,followed by control diet(florfenicol group,F1)for 15 d;diet sprayed with florfenicol for 15 d,then diet sprayed with L.plantarum for 15 d,and then diet with control diet for 15 d(L.plantarum and florfenicol group,G1).The Sebastes schlegelii were dissected and took intestines in the 30th and the 45th day,and the changes of intestinal histomorphological structure were observed by HE staining and Image J software.Changes inα-amylase,lipase,trypsin,alkaline phosphatase,and superoxide dismutase were measured in serum.The results showed that the length of intestinal villi,the thickness of muscular layer and the depth of crypt(P<0.05)were observed in the fed diets containing 1×10^(7)CFU/g L.plantarum and 20 mg/L florfenicol.The activities ofα-amylase,alkaline phosphatase and superoxide dismutase were significantly increased in the fed diet containing 1×10^(7)CFU/g L.plantarum LP-37 can(P<0.05).The activity ofα-amylase,trypsin,alkaline phosphatase and superoxide dismutase were significantly increased by florfenicol at 20 mg/L,and the activity of lipase was significantly decreased(P<0.05).Combination 1×10^(7)CFU/g L.plantarum LP-37 and 20 mg/L florfenicol led to significantly increaseα-amylase activity and superoxide dismutase activity(P<0.05)significantly decreased lipase and trypsin activity(P<0.05).To sum up,L.plantarum LP-37 and florfenicol improved the intestinal structure of Sebastes schlegelii and regulated the digestive and immune enzyme activities of Sebastes schlegelii.

关 键 词：许氏平鲉 肠道形态 消化酶 免疫酶 植物乳杆菌 氟苯尼考 

分 类 号：S965.399[农业科学—水产养殖]