Molecular Cloning and Bioinformatics Analysis of sucC Gene of Vibrio alginolyticus Strain HY9901  

作　　者：Yingzhu WEI Zhiqing WEI Xuelian LIN Huanying PANG Na WANG 

机构地区：[1]Fisheries College,Guangdong Ocean University,Zhanjiang 524025,China [2]Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture&Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes,Zhanjiang 524025,China [3]Chinese Academy of Inspection and Quarantine,Beijing 100176,China

出　　处：《Asian Agricultural Research》2024年第8期32-37,共6页亚洲农业研究（英文）

基　　金：Supported by National Natural Science Foundation of China(32073015);Graduate Education Innovation Program of Guangdong Province(YJYH[2022]1);Undergraduate Innovation and Entrepreneurship Training Program of Guangdong Ocean University(CXXL2024007);Undergraduate Innovation Team of Guangdong Ocean University(CCTD201802).

摘　　要：[Objectives]To clone the sucC gene of Vibrio alginolyticus strain HY9901 and conduct the bioinformatics analysis.[Methods]Based on the sucC gene of V.alginolyticus strain HY9901,specific primers were designed to amplify the full length sequence by PCR and make further analysis.[Results]The theoretical molecular weight of SucC protein was about 41528.45 Da,and the full length was 1167 bp,encoding 388 amino acids.It has no signal peptide and transmembrane region,and has a variety of functional sites.It is predicted that it is mainly located in the cytoplasm,and the ubiquitin and lactate modification sites overlap,and it has high gene homology with Vibrio parahaemolyticus.Theα-helix,random coil and extended strand are the main secondary structures.The similarity between the constructed three-level structure model and the template is high.[Conclusions]This study reveals the structural characteristics and functional potential of SucC protein,and provides a theoretical basis for the study of drug resistance mechanism and prevention strategies.

关 键 词：VIBRIO ALGINOLYTICUS GENE amplification sucC GENE Succinyl-Coa SYNTHETASE Protein POST-TRANSLATIONAL modification Bioinformatics analysis 

分 类 号：Q78[生物学—分子生物学] Q94