负载BMP-2 壳聚糖水凝胶在大鼠完全脱位牙再植愈合中的研究  

Healing effect of BMP-2-loaded chitosan hydrogel on the replantation of completely dislocated teeth in rats

作　　者：胡通 张珏 朱秀安 苑迎娇 HU Tong;ZHANG Jue;ZHU Xiuan;YUAN Yingjiao(School of Stomatology,Wannan Medical College,Wuhu 241002,Anhui,China)

机构地区：[1]皖南医学院口腔医学院,安徽芜湖241002 [2]皖南医学院第一附属医院弋矶山医院口腔科,安徽芜湖241001

出　　处：《皖南医学院学报》2024年第4期361-364,共4页Journal of Wannan Medical College

基　　金：皖南医学院中青年科研基金项目(WK202006)。

摘　　要：目的:研究负载骨形态发生蛋白(BMP-2)壳聚糖水凝胶在大鼠脱位牙再植愈合中的成骨作用。方法:24只SD大鼠随机分为4组。A组为空白对照组,牙槽窝仅注入生理盐水;B组为实验对照组1,牙槽窝注入壳聚糖水凝胶;C组为实验对照组2,牙槽窝注入BMP-2试剂;D组为实验组,牙槽窝注入负载BMP-2的壳聚糖水凝胶。全麻下完整拔出大鼠上颌右侧中切牙,刮除牙周膜,拔除牙髓,根尖孔打入vitapex,玻璃离子封闭根尖孔,4组分别注入设定的试剂然后再植入牙槽窝并固定。2个月后取带有上颌前牙的牙槽骨,脱钙后包埋、切片、HE染色,光镜下观察各组样本中牙周膜愈合、骨性愈合、炎症性吸收的分值,应用SPSS 24.0软件对数据进行统计处理。结果:D组的牙周膜愈合分值高于A、B、C组,差异均有统计学意义(P<0.05);D组的炎症吸收分值低于A、B、C组,差异均有统计学意义(P<0.05),其余组间牙周膜愈合与炎症性吸收两两比较差异无统计学意义(P>0.05)。结论:负载BMP-2壳聚糖水凝胶能有效促进大鼠脱位牙再植后牙周缺损的修复。Objective:To investigate the osteogenic effect of BMP-2-loaded chitosan hydrogel in the healing process of replanted dislocated teeth in rats.Methods:Twenty-four SD rats were randomly divided into 4 groups.Group A served as blank control group,in which rats were injected with normal saline into the alveolar sockets.Group B and C were experimental controls.Group B received an injection of chitosan hydrogel,and Group C received BMP-2 solution.Group D,the experimental group,was administered with BMP-2-loaded chitosan hydrogel.Under general anesthesia,the right maxillary central incisors of rats were completely extracted,followed by removal of the periodontal ligament and dental pulp.Vitapex was injected into the apical foramen,which was then sealed with glass ionomer.The four groups were injected with the set reagents respectively,and then the teeth were implanted into the alveolar sockets and fixed.After two months,the alveolar bone with maxillary anterior teeth was retrieved,decalcified,embedded,sectioned,and stained with HE.The periodontal ligament healing,osseous healing,and inflammatory resorption in each group of samples were observed under an electron microscope.The data were statistically processed using SPSS 24.0 software.Results:The periodontal ligament healing score of Group D was significantly higher than that of Groups A,B,and C.Its inflammatory resorption score was significantly lower.These differences were also statistically significant(P<0.05).No significant differences were observed in periodontal ligament healing and inflammatory resorption between other groups(P>0.05).Conclusion:BMP-2-loaded chitosan hydrogel can effectively promote the repair of periodontal defects after replantation of dislocated teeth in rats.

关 键 词：牙周膜干细胞 骨形态发生蛋白-2 壳聚糖 水凝胶 

分 类 号：R-332[医药卫生] R783.6