牡荆素抗肥胖作用研究及其作用机制初探  

A preliminary study on the anti-obesity effect of vitexin and its mechanism of action

作　　者：姚子晴 沈长虹 张若琪[1] YAO Ziqing;SHEN Changhong;ZHANG Ruogi(School of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,Sichuan)

机构地区：[1]成都中医药大学药学院,四川成都610075

出　　处：《中药与临床》2024年第3期68-74,92,共8页Pharmacy and Clinics of Chinese Materia Medica

基　　金：四川省自然科学基金(2022NSFSC1349);成都中医药大学杏林学者基金(QJRC2022023)。

摘　　要：目的:本研究旨在验证牡荆素对肥胖小鼠的疗效,并通过网络药理学与分子对接对其作用机制进行初步的研究。方法:通过给小鼠喂食高脂饲料造小鼠肥胖模型,随后喂食高脂饲料的同时给予牡荆素,对小鼠的体重进行称量,测定肝脏中的TC和TG含量,对肝脏和脂肪细胞进行H&E染色观察;利用网络药理学技术分析牡荆素治疗肥胖的关键通路。结果:使用高脂饲料喂养的小鼠在第4周与空白对照组之间存在明显的体重差异,表明造模成功;在给予牡荆素(3×10^(-2)mg·g^(-1))5周后,牡荆素组小鼠的体重增长趋势得到了显著的减缓;血脂水平的表明牡荆素能够减少肥胖小鼠肝脏中的TG和TC含量,从而降低肝脏脂肪滴并抑制脂肪细胞的增长。网络药理学KEGG富集结果显示PI3K-AKT通路可能是关键通路。结论:研究通过小鼠肥胖模型证实牡荆素在治疗肥胖方面的有效性。利用网络药理学和分子对接技术,发现牡荆素治疗肥胖的作用机制可能是通过PI3K/AKT等途径来实现的,这为膳食补充剂在肥胖治疗方面提供了有价值的数据参考。Objective:The aim of this study was to verify the efficacy of vitexin in obese mice and to conduct a preliminary study of its mechanism of action using network pharmacology and molecular docking technology.Methods:Mice were fed a high-fat diet to create a mouse obesity model and then given vitexin at the same time as the high-fat diet.The body weight of the mice was weighed,the TC and TG content of the liver was determined,and the liver and adipocytes were observed by H&E staining.Network pharmacology was used to analyze the key pathways of vitexin in the treatment of obesity.Results:There was a significant weight difference between the mice fed the high-fat diet and the blank control group after 4 weeks,indicating that the modelling was successful.The weight gain trend of the mice in the vitexin group was significantly slowed down after 5 weeks of vitexin(3×10^(-2)mg·g^(-1))administration.The blood lipid level showed that vitexin could reduce the TG and TC content in the liver of obese mice,thereby reducing the liver fat droplets and inhibiting the growth of fat cells.The KEGG enrichment results of network pharmacology showed that the PI3K-AKT pathway may be the key pathway.Conclusion:Studies have confirmed the efficacy of vitexin in the treatment of obesity using a mouse model of obesity.Using network pharmacology and molecular docking technology,it is found that the mechanism of action of vitexin in the treatment of obesity may be realized through PI3K/AKT and other pathways,which provides a valuable data reference for dietary supplements in the treatment of obesity.

关 键 词：牡荆素 肥胖 网络药理学 分子对接 

分 类 号：R285.5[医药卫生—中药学]