基于中红外光谱技术快速检测生乳中的腐胺  

Rapid Detection of Putrescine in Raw Milk Based on Mid-Infrared Spectroscopy Technology

作　　者：佀博学 张养东[1,2] 郑楠[1,2] 王加启[1,2] SI Boxue;ZHANG Yangdong;ZHENG Nan;WANG Jiaqi(Key Laboratory of Quality&Safety Control for Milk and Dairy Products of Ministry of Agriculture and Rural Affairs,Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing 100193,China;State Key Laboratory of Animal Nutrition and Feeding,Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,China)

机构地区：[1]中国农业科学院北京畜牧兽医研究所,农业农村部奶及奶制品质量安全控制重点实验室,北京100193 [2]中国农业科学院北京畜牧兽医研究所,畜禽营养与饲养全国重点实验室,北京100193

出　　处：《动物营养学报》2024年第8期5432-5440,共9页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家重点研发计划(2022YFD1301004);中国农业科学院科技创新工程(ASTIP-IAS12);国家奶牛产业技术体系(CARS-36)。

摘　　要：本试验旨在通过建立中红外光谱预测模型实现生乳中腐胺的快速检测。将采集到的16个牧场生乳样品在4℃条件下贮藏0~7 d,使用高效液相色谱-质谱法定量检测生乳中腐胺含量;通过FT 120乳成分分析仪采集生乳的中红外光谱,将原始中红外光谱先通过标准正态变量变换(SNV)、Savitsky-Golay(SG)平滑、一阶导数或二阶导数等预处理方法消除背景噪音,再利用偏最小二乘回归(PLSR)法构建腐胺预测模型。结果显示:通过比较不同中红外光谱预处理方法,最终选择SG平滑+二阶导数预处理光谱建立腐胺预测模型,该模型测试集决定系数(R^(2))为0.9959,均方根误差(RMSE)为0.0542,验证集R^(2)为0.8112,RMSE为0.3684,具有良好的准确性。本试验基于中红外光谱建立了生乳中腐胺快速检测方法,可为生乳中腐胺的快速检测提供参考。The aim of this experiment was to establish a mid-infrared spectral prediction model for the rapid detection of putrescine in raw milk.Raw milk samples collected from 16 farms were stored at 4℃for 0 to7 d.The content of putrescine in raw milk was detected by high performance liquid chromatography-mass spectrometry method,and the mid-infrared spectrum of raw milk was collected by FT 120 milk composition analyzer.The original mid-infrared spectrum was preprocessed by standard normal variate transformation(SNV),Savitsky-Golay(SG)smoothing,first-order derivative or second-order derivative to eliminate background noise,and then partial least squares regression(PLSR)method was used to construct the putrescine prediction model.The results showed that by comparing different mid-infrared spectral preprocessing methods,the SG smoothing+second-order derivative preprocessing method was finally chosen to preprocess the spectra to establish a prediction model,the model has an determination coefficient(R^(2))of 0.9959 and an root mean square error(RMSE)of 0.0542 for the test set and an R^(2) of 0.8112 and an RMSE of 0.3684 for the validation set,providing good accuracy and stability.In conclusion,a rapid method for the determination of putrescine in raw milk is established based on mid-infrared spectroscopy,which can provide reference for the rapid detection of putrescine in raw milk.

关 键 词：中红外光谱 生乳 腐胺 偏最小二乘回归 

分 类 号：S816[农业科学—饲料科学]