miR-107对口腔鳞癌细胞系CAL27增殖、侵袭及迁移的影响  

Effects of miR-107 on proliferation,invasion and migration of CAL27 cells in oral squamous cell carcinoma

作　　者：张晋玮[1] 王燕 王通 ZHANG Jinwei;WANG Yan;WANG Tong(Department of Stomatology,Branch of Tianjin Third Central Hospital,Tianjin 300250,China;Department of Rehabilitation,the Worker's Sanatorium Hospital of Ningxia;Department of Stomatology,Tianjin First Central Hosptial)

机构地区：[1]天津市第三中心医院分院口腔科,300250 [2]宁夏工人疗养院康复科 [3]天津市第一中心医院口腔科

出　　处：《天津医药》2024年第9期897-900,共4页Tianjin Medical Journal

基　　金：国家自然科学基金资助项目(82173380)。

摘　　要：目的探讨miR-107在口腔鳞癌细胞系CAL27中的表达及其对CAL27细胞增殖、凋亡、侵袭和迁移的影响。方法采用实时荧光定量PCR检测miR-107在口腔鳞癌细胞株CAL27和人口腔上皮细胞HOEC细胞中的表达情况;脂质体转染法将miR-107 mimic和miR-107 NC质粒分别转染进入CAL27细胞,并分为对照(miR-107 NC)组和miR-107过表达(miR-107 mimic)组;MTT细胞增殖实验检测细胞增殖能力,流式细胞术检测细胞凋亡率,Transwell小室实验检测细胞迁移和侵袭能力。结果CAL27细胞系中miR-107的表达低于HOEC细胞。miR-107 mimic组细胞转染后24、48、72 h光密度(OD)值均低于miR-107 NC组,凋亡细胞比例高于miR-107 NC组,而侵袭和迁移细胞数少于miR-107 NC组(P<0.05)。结论上调miR-107可抑制CAL27细胞的增殖、侵袭和迁移,并促进其凋亡。Objective To investigate the expression miR-107 in oral squamous cell carcinoma CAL27 cell line and its effect on the proliferation,apoptosis,invasion and migration of CAL27 cells.Methods The expression of miR-107 CAL27 cell line and human oral epithelial HOEC cells were detected by real-time quantitative fluorescent PCR(qRT-PCR).MiR-107 mimic and miR-107 NC plasmid were transfected into CAL27 cells using liposome transfection method,and cells were divided into two groups,the miR-107 NC group used as a control group and the miR-107 mimic group used as a miR-107-overexpressed group.MTT cell proliferation assay was used to detect cell proliferation.Flow cytometry was used to detect cell apoptosis.Transwell chamber assay was used to detect cell migration and invasion.Results The expression of miR-107 was lower in CAL27 cell line than that in HOEC cells.OD values of the miR-107 mimic group were lower than those of the miR-107 NC group at the time of 24,48 and 72 h after transfection.The proportion of apoptotic cells was higher in the miR-107 mimic group than that of the miR-107 NC group,while the number of invasion and migration cells was lower than that of the miR-107 NC group(P<0.05).Conclusion Up-regulation of miR-107 can inhibit proliferation,invasion and migration of CAL27 cells.

关 键 词：口腔肿瘤 RNA干扰疗法 细胞增殖 肿瘤浸润 细胞运动 miR-107 

分 类 号：R739.85[医药卫生—肿瘤]