结直肠癌组织LncRNA LINC00342和miR-203a-3p表达及与预后的关系  

Expression of LncRNA LINC00342 and miR-203a-3p in colorectal cancer tissue and their relationship with prognosis

作　　者：王新波[1] 罗冰清[1] 石玉宝[1] 张也 席江伟[1] WANG Xinbo;LUO Bingqing;SHI Yubao;ZHANG Ye;XI Jiangwei(Department of General Surgery,the Second Hospital Affiliated to Hebei North University,Zhangjiakou 075100,China)

机构地区：[1]河北北方学院附属第二医院普通外科,075100

出　　处：《天津医药》2024年第9期971-976,共6页Tianjin Medical Journal

基　　金：河北省医学科学研究课题计划项目(20220496)。

摘　　要：目的分析结直肠癌组织长链非编码RNA(LncRNA)LINC00342、微小RNA-203a-3p(miR-203a-3p)表达水平与患者术后5年内预后的关系。方法采集133例结直肠癌患者的结直肠癌组织及癌旁组织。荧光定量PCR法检测LncRNA LINC00342和miR-203a-3p表达;术后随访5年记录患者生存和死亡情况。比较不同情况下LncRNA LINC00342、miR-203a-3p表达及临床病理参数。分析结直肠癌组织中LncRNA LINC00342、miR-203a-3p表达的相关性、与预后的关系及对预后的预测价值。结果结直肠癌组织中LncRNA LINC00342表达水平高于癌旁组织,miR-203a-3p表达水平低于癌旁组织(P<0.05)。结直肠癌组织中LncRNA LINC00342与miR-203a-3p表达水平呈负相关(P<0.05)。LncRNA LINC00342高表达组、miR-203a-3p低表达组肿瘤低分化、TNMⅢ期、有淋巴结转移患者比例分别高于LncRNA LINC00342低表达组和miR-203a-3p高表达组(P<0.05)。LncRNA LINC00342高表达组、miR-203a-3p低表达组术后5年总生存率更低(P<0.05)。死亡组肿瘤低分化、TNMⅢ期、有淋巴结转移患者比例、LncRNA LINC00342表达水平高于存活组,miR-203a-3p表达水平低于存活组(P<0.05)。肿瘤低分化、TNMⅢ期、有淋巴结转移、LncRNA LINC00342高表达、miR-203a-3p低表达是影响患者术后5年内死亡的独立危险因素(P<0.05)。LncRNA LINC00342和miR-203a-3p联合对预后的预测价值高于单独预测。结论结直肠癌组织中LncRNA LINC00342呈高表达,miR-203a-3p呈低表达,二者联合检测有望成为预测术后生存的临床评估指标。Objective To analyze the relationship between expression levels of long non-coding RNA(LncRNA)LINC00342 and microRNA-203a-3p(miR-203a-3p)in colorectal cancer tissue and the prognosis of patients within 5 years after surgery.Methods Samples of colorectal cancer tissue and paracancer tissue of 133 patients with colorectal cancer were collected.Expression levels of LncRNA LINC00342 and miR-203a-3p were detected by fluorescence quantitative PCR.Survival and death were recorded after 5 years of follow-up.The LncRNA LINC00342 and miR-203a-3p expressions and clinicopathological parameters in patients under different conditions were compared.The correlation between expressions of LncRNA LINC00342 and miR-203a-3p in colorectal cancer tissue was analyzed,and their predictive value for the prognosis was also analyzed.Results The expression level of LncRNA LINC00342 was higher in colorectal cancer tissue than that in paracancer tissue,and the expression level of miR-203a-3p was lower in colorectal cancer tissue than that in paracancer tissue(P<0.05).The expression levels of LncRNA LINC00342 and miR-203a-3p in colorectal cancer tissue were negatively correlated(P<0.05).The proportion of patients with low tumor differentiation,TNM stageⅢand lymph node metastasis were higher in the LncRNA LINC00342 high expression group and the miR-203a-3p low expression group than those in the LncRNA LINC00342 low expression group and the miR-203a-3p high expression group,respectively(P<0.05).The 5-year overall survival rate after operation was lower in the LncRNA LINC00342 high expression group and the miR-203a-3p low expression group(P<0.05).The proportion of patients with low tumor differentiation,TNM stageⅢ,lymph node metastasis,and higher expression level of LncRNA LINC00342 were higher in colorectal cancer tissue of the death group than those in the survival group,and the expression level of miR-203a-3p was lower than that in the survival group(P<0.05).Low tumor differentiation,TNM stageⅢ,lymph node metastasis,high expression of Ln

关 键 词：结直肠肿瘤 RNA 长链非编码 预后 长链非编码RNA LINC00342 微小RNA-203a-3p 

分 类 号：R735.34[医药卫生—肿瘤]