基于RNA测序和生物信息学分析鉴定椎旁肌退变中关键的铁死亡基因  

Identification of key ferroptosis genes in paraspinal muscle degeneration based on RNA sequencing and bioinformatics analysis

作　　者：张春虹[1] 黄洪超[1] 刘越[1] 杜立龙 许海委[1] 黎宁[1] 李勇进 ZHANG Chunhong;HUANG Hongchao;LIU Yue;DU Lilong;XU Haiwei;LI Ning;LI Yongjin(Department of Minimally Invasive Spine Surgery,Tianjin Hospital,Tianjin 300211,China;Department of Spine Surgery,the First Affiliated Hospital of University of Science and Technology of China)

机构地区：[1]天津市天津医院微创脊柱外科,300211 [2]中国科学技术大学附属第一医院脊柱外科

出　　处：《天津医药》2024年第9期991-995,共5页Tianjin Medical Journal

基　　金：天津市卫生健康委员会科技项目(TJWJ2023QN052)。

摘　　要：目的探究椎旁肌退变(PMD)中的基因表达谱,并鉴定关键的铁死亡基因。方法选取正常和PMD患者各3例,分别取椎旁肌组织进行RNA测序,获得差异表达的基因。通过蛋白-蛋白相互作用(PPI)和基因功能富集分析,与铁死亡基因取交集,鉴定与铁死亡相关的关键中枢基因。通过受试者工作特征(ROC)曲线分析铁死亡基因对PMD疾病的诊断价值。结果在PMD中共鉴定出292个差异表达的基因,其中125个显著下调,167个显著上调。生物信息学分析发现14个差异表达的基因与铁死亡相关,其中铁死亡基因MUC1、ATF3、CDKN1A是关键的中枢基因,对诊断PMD具有良好的敏感度和特异度。功能富集分析发现它们可能通过调控细胞凋亡、铁死亡和骨骼肌组织发育、分化等介导PMD的发生与进展。结论铁死亡基因MUC1、ATF3、CDKN1A可作为诊断PMD的生物标志物,为解码PMD的病理机制及开发新的药物提供理论依据。Objective To explore the gene expression profile in paraspinal muscle degeneration(PMD)and identify key ferroptosis genes.Methods RNA sequencing was performed on paraspinal muscle tissue of 3 normal and 3 PMD patients respectively to obtain differentially expressed genes.Through protein-protein interaction(PPI)and gene functional enrichment analysis,the intersection of ferroptosis genes was identified to identify key hub genes associated with ferroptosis.The diagnostic value for PMD disease was analyzed by receiver operating characteristic(ROC)curves.Results A total of 292 differentially expressed genes were identified in PMD.Among them,125 genes were significantly downregulated and 167 genes were significantly upregulated.Bioinformatics analysis revealed that 14 differentially expressed genes were associated with ferroptosis.Among them,ferroptosis genes MUC1,ATF3 and CDKN1A were key hub genes with good specificity and sensitivity for diagnosing PMD.Functional enrichment analysis revealed that they may mediate the occurrence and progression of PMD by regulating cell apoptosis,ferroptosis and skeletal muscle tissue development and differentiation.Conclusion Ferroptosis genes MUC1,ATF3 and CDKN1A can serve as biomarkers for diagnosing PMD,providing theoretical basis for decoding the pathological mechanism of PMD and developing new drugs.

关 键 词：序列分析 RNA 铁死亡 计算生物学 椎旁肌退变 

分 类 号：R685[医药卫生—骨科学]