白木香叶中5种功效成分的同步测定及其指纹图谱的构建  

Construction the Method of Five Functional Components Simultaneous Determination and Fingerprint in Aquilaria sinensis Leaves

作　　者：杨柳 娄涛 陈颖乐 段金龙 王颂 曾雷[1] 谭建文 徐巧林[1] YANG Liu;LOU Tao;CHEN Yingle;DUAN Jinlong;WANG Song;ZENG Lei;TAN Jianwen;XU Qiaolin(Guangdong Provincial Key Laboratory of Silviculture,Guangdong Academy of Forestry,Guangzhou 510650,China;South China Agricultural University,Guangzhou 510642,China)

机构地区：[1]广东省森林培育与保护利用重点实验室,广东省林业科学研究院,广东广州510650 [2]华南农业大学,广东广州510642

出　　处：《食品科技》2024年第7期283-289,共7页Food Science and Technology

基　　金：广东省林业科技计划项目(2021KYXM01);广东省林业科技创新项目(2020KJCX013)。

摘　　要：文章建立白木香叶的高效液相色谱指纹图谱及其活性成分含量的同步测定方法,为白木香叶的质量控制及开发利用提供科学依据。对提取溶剂和色谱条件进行优化,采用100%甲醇提取,Phenomenex-C_(18)色谱柱,甲醇-纯水为流动相梯度洗脱,检测波长254 nm。对优化后的方法进行系统的方法学验证,对羟基苯甲酸、腺苷、芒果苷、木犀草素和丹参酮Ⅰ等5种成分线性关系良好(r≥0.9992),加样回收率为96.53%~98.71%,回收率高,精密度好。建立32批白木香叶指纹图谱,相似度为0.889~0.998。该方法通过对5种功效成分含量的同步测定,直观反映出白木香叶的化学成分差异,为华南地区白木香叶产业的质量评价标准的建立提供了参考。The high performance liquid chromatographic(HPLC)fingerprint of Aquilaria sinensis leaves and the simultaneous determination of its active ingredient content were established to provide scientific evidence for the quality control and exploitation of Aquilaria sinensis leaves.The extraction solvent and chromatographic conditions were optimized with 100%methanol extraction,Phenomenex-C18column,methanol-pure water as mobile phase gradient elution,and the detection wavelength of 254nm.The optimized method was systematically validated,and the linearity of the five constituents,including p-hydroxybenzoic acid,adenosine,arnica glycoside,lignoceroside,and tanshinoneⅠ,was good(r≥0.9992),and the spiked recoveries were 96.53%–98.71%.The recoveries were high and the precision was good.The fingerprints of 32 batches of Aquilaria sinensis leaves were established,and the similarities were 0.889–0.998.The method intuitively reflected the differences in the chemical composition of Aquilaria sinensis leaves through the simultaneous determination of the contents of the five active ingredients,and provided a reference for the establishment of the quality evaluation standards for the industry of Aquilaria sinensis leaves industry in South China.

关 键 词：白木香叶 指纹图谱 含量测定 不同产地 质量评价 

分 类 号：TS207.3[轻工技术与工程—食品科学]