基于适配体-悬浮芯片技术检测黄瓜中毒死蜱残留  

Detection of Chlorpyrifos Residue in Cucumber Based on Aptamer-Suspension Chip

作　　者：龙彩云[1] 赵海玲[1] 雷琼[1] 何娟 刘慧 何圣发 LONG Caiyun;ZHAO Hailing;LEI Qiong;HE Juan;LIU Hui;HE Shengfa(Ganzhou Center for Disease Control and Prevention,Ganzhou 341000,China;School of Public Health and Healthcare Management,Gannan Medical University,Ganzhou 341000,China)

机构地区：[1]赣州市疾病预防控制中心,江西赣州341000 [2]赣南医科大学公共卫生与健康管理学院,江西赣州341000

出　　处：《食品科技》2024年第7期330-336,共7页Food Science and Technology

基　　金：江西省卫生健康委员会科技计划项目(20212006)。

摘　　要：文章开发了一种基于适配体-悬浮芯片的技术,用于快速检测黄瓜中毒死蜱残留。将毒死蜱适配体与荧光微球共价偶联作为检测探针,以生物素修饰的适配体3'端互补链为竞争探针,在反应体系中毒死蜱和竞争探针共同竞争结合检测探针上的适配体,竞争探针上的生物素可以与链霉亲和素标记的荧光藻红蛋白特异性结合,使互补链带有荧光。毒死蜱含量与荧光强度成反比,从而通过荧光强度分析样品中的毒死蜱含量。在优化条件下,该方法对毒死蜱表现出高灵敏度和高特异性,检测浓度范围为0.039~234.63 ng/mL,相关系数R^(2)为0.98,检出限为0.014 ng/mL。对黄瓜的加标回收率为92.00%~105.30%,相对标准偏差为1.76%~4.13%。该方法具有高通量、高灵敏度、好的特异性等优点,在毒死蜱残留快速检测方面具有良好的应用潜力。A method based on aptamer-suspension chip has been developed for the rapid detection of chlorpyrifos residues in cucumber.The aptamers of chlorpyrifos were covalently bound to carboxylated fluorescent microspheres to serve as detection probe,the 3'end complementary chains modified by biotin were used as competitive probe.The biotin-modified complementary chains could specifically bind streptavidin,R-phycoerythrin conjugates,resulting the complementary chain fluorescent and the content of chlorpyrifos was inversely proportional to the fluorescence intensity.Therefore,the content of chlorpyrifos in samples can be evaluated by fluorescence intensity.Under the optimized conditions,the developed method showed high sensitivity and specificity for chlorpyrifos.The detection concentration ranged from 0.039 ng/mL to 234.63 ng/mL,with a good linear connection(R^(2)=0.98)and low limit of detection 0.014ng/mL.The recovery of cucumber and the relative standard deviation were in ranges of 92.00%–105.30%and 1.76%–4.13%.The method exhibited high throughput,high sensitivity and good specificity,and had good application potential in the rapid detection of chlorpyrifos residues.

关 键 词：毒死蜱 适配体 互补链 悬浮芯片 

分 类 号：TS207.5*3[轻工技术与工程—食品科学]