白酒糟中纤维素酶解工艺优化研究  

Optimization of enzymatic hydrolysis process for cellulose in distiller's grain waste

作　　者：徐健 赵雨 胡芳 刘建忠 程润喜 汪江波 XU Jian;ZHAO Yu;HU Fang;LIU Jian-zhong;CHENG Run-xi;WANG Jiang-bo(Key Laboratory of Fermentation Engineering(the Ministry of Education),Hubei Provincial Key Laboratory of Industrial Microbiology,Cooperative Innovation Center of Industrial Fermentation(the Ministry of Education&Hubei Province),School of Biological Engineering and Food,Hubei University of Technology,Wuhan 430000,China;Sichuan Provincial Key Laboratory of Liquor Brewing Biotechnology and Application,Sichuan University of Science&Engineering,Yibin 644000,China;Road Environment Technology Co.,Ltd.,Wuhan 430000,China)

机构地区：[1]工业发酵省部共建协同创新中心,发酵工程教育部重点实验室,工业微生物湖北省重点实验室,湖北工业大学生物工程与食品学院,湖北武汉430000 [2]酿酒生物技术及应用四川省重点实验室,四川轻化工大学,四川宜宾644000 [3]路德环境科技股份有限公司,湖北武汉430000

出　　处：《现代化工》2024年第9期155-159,166,共6页Modern Chemical Industry

基　　金：湖北省重点研发计划项目(2021BGD016);湖北省知识产权局项目(ZXKY2022498);酿酒生物技术及应用四川省重点实验室开放课题(NJ2022-03)。

摘　　要：为实现白酒糟资源的综合利用,探究了白酒糟渣中纤维素酶解的最佳工艺参数。以可发酵糖得率为评价指标,通过单因素实验探究了预处理条件(固液比、硫酸体积分数、处理温度、处理时间)及酶解条件(酶添加量)对酒糟中纤维素酶解效果的影响,在此基础上用神经网络分析结合遗传算法进行进一步优化。结果表明,白酒糟纤维素的最佳酶解工艺参数为:固液比为1∶10、硫酸体积分数为7%、温度为170℃、时间为40 min、酶添加量为23 FPU/g。此条件下酒糟纤维素酶解得到的可发酵糖总量为(19.77±0.56) g/L,得率为88.97%。以该酶解液作为碳源进行丁醇发酵,溶剂(丁醇、乙醇、异丙醇和丙酮)总产量为1.56 g/L。The optimum technological parameters for enzymatic hydrolysis of cellulose in distiller's grains waste(DGW)are explored to realize the comprehensive utilization of DGW resources.Taking the yield of fermentable sugars as the evaluation index,the influences of pretreatment conditions(solid-liquid ratio,sulfuric acid volume fraction,treatment temperature,and treatment time)and enzymatic hydrolysis conditions(enzyme addition amount)on the enzymatic hydrolysis of cellulose in DGW are respectively investigated through single factor experiment.On this basis,both neural network analysis and genetic algorithm are employed to optimize the enzymatic hydrolysis process.As a result,the optimum parameters for enzymatic hydrolysis of cellulose in DGW are as follows:solid-liquid ratio is 1∶10(w/v),sulfuric acid concentration is 7%(v/v),temperature is 170℃,time is 40 min,and enzyme dosage is 23 FPU·g^(-1).Under these conditions,the total amount of fermentable sugars obtained by cellulose hydrolysis is 19.77±0.56 g·L^(-1),and the yield is 88.97%.Additionally,total yield of solvent(butanol,ethanol,isopropyl alcohol,and acetone)is 1.56 g·L^(-1) when the enzymatic hydrolysis solution as carbon source is conducted for butanol fermentation.

关 键 词：白酒糟 纤维素酶解 工艺优化 神经网络分析 丁醇发酵 

分 类 号：TQ353.9[化学工程]