安石榴苷对溃疡性结肠炎小鼠肠道菌群和炎性因子的影响  

Effects of punicalagin on intestinal microbiota and inflammatory factors in mice with ulcerative colitis

作　　者：范高福[1] 孙莉华 杨新华 汤洁[1] 韦梦强 FAN Gaofu;SUN Lihua;YANG Xinhua;TANG Jie;WEI Mengqiang(School of Biomedical Engineering,Hefei Technology College,Hefei,Anhui 238000,China;不详)

机构地区：[1]合肥职业技术学院生物工程学院,安徽合肥238000 [2]合肥市第八人民医院药剂科,安徽合肥238000

出　　处：《中国微生态学杂志》2024年第7期769-775,共7页Chinese Journal of Microecology

基　　金：安徽省高校自然科学研究重大项目(KJ2021ZD0159)。

摘　　要：目的探究安石榴苷对溃疡性结肠炎(UC)小鼠肠道菌群和炎性因子的影响。方法实验小鼠分为对照组(Con组)、UC模型组(UC组)、5-氨基水杨酸治疗组(5-ASA组)和安石榴苷干预组(Pun组),每组各5只小鼠。观察小鼠体重、便血和粪便形状,并进行疾病活动指数(DAI)评分;通过HE染色观察小鼠结肠组织切片形态,并进行病理评分与统计;ELISA法检测小鼠血清IL-6、TNF-α、IL-1β、IL-8和IL-10水平;16S rRNA高通量测序检测小鼠肠道菌群。结果与UC组相比,Pun组小鼠DAI评分显著降低;HE结果提示安石榴苷可改善UC小鼠结肠损伤、恢复肠道腺体和隐窝结构、减少炎性细胞浸润;Pun组小鼠血清促炎因子IL-6、IL-8、IL-1β和TNF-α水平显著降低(P<0.01),而抑炎因子IL-10水平显著增加(P<0.001)。测序结果显示,安石榴苷可提高小鼠肠道菌群多样性,增加Prevotellaceae_UCG-001和乳酸杆菌属(Lactobacillus)相对丰度,降低阿克曼菌属(Akkermansia)和联合乳酸杆菌属(Ligilactobacillus)相对丰度。结论安石榴苷可有效缓解小鼠UC结肠炎症状,通过降低血清炎症因子水平和调节肠道菌群平衡达到治疗效果,这为UC的临床治疗提供新思路。Objective To observe the impact of punicalagin on intestinal microbiota and inflammatory factors in mice with ulcerative colitis(UC).Methods The mice were divided into four groups,namely control group(Con),UC model group(UC),5-aminosalicylic acid treatment group(5-ASA),and punicalagin administration group(Pun),5 mice in each group.After administered punicalagin,the body weight,stool blood,and stool shape of the mice were observed,and the disease activity index(DAI)scores were determined.Hematoxylin-eosin(HE)staining was used to observe colon tissue sections for morphological analysis,pathological scoring,and statistical valuation.Serum levels of IL-6,TNF-α,IL-1β,IL-8 and IL-10 were measured using ELISA.Intestinal microbiota of the mice were analyzed through 16S rRNA highthroughput sequencing.Results Compared to the UC model group,the Pun group exhibited a significant decrease in DAI score.HE results indicated that punicalagin improved colon injury by restoring intestinal glands and crypt structure while reducing inflammatory cell infiltration in UC mice.Serum levels of proinflammatory cytokines IL-6,IL-8,IL-1βand TNF-αsignificantly decreased in the Pun group(P<0.01),whereas that of anti-inflammatory cytokine IL-10 significantly increased(P<0.001).Analysis of sequencing data revealed that punicalagin enhanced intestinal microbiota diversity while increasing relative abundances of Prevotellaceae_UCG-001 and Lactobacillus but decreasing those of Akkermansia and Ligilactobacillus.Conclusion Punicalagin can effectively alleviate the symptoms of UC in mice,and achieve the therapeutic effect by reducing serum inflammatory factor levels and regulating intestinal microbiota balance.This provides new ideas for the clinical treatment of UC.

关 键 词：安石榴苷 溃疡性结肠炎 肠道菌群 16S rRNA高通量测序 

分 类 号：R574.1[医药卫生—消化系统]