口腔微生物影响放射性口炎:一项单臂前瞻性研究  

Impact of oral microbiota on radiation⁃induced oral mucositis:A single⁃arm prospective study

作　　者：卢志远 许益敏 周梦缘 卢杨 魏志豪 肖灿[1] LU Zhiyuan;XU Yimin;ZHOU Mengyuan;LU Yang;WEI Zhihao;XIAO Can(Department of Stomatology,the First Affiliated Hospi-tal of Soochow University,tate Key Laboratory of Radiation Medicine and Protection,Soochow University,Suzhou 215026,China)

机构地区：[1]苏州大学附属第一医院口腔科,苏州大学放射医学与辐射防护国家重点实验室,江苏苏州215026

出　　处：《口腔医学》2024年第8期596-601,共6页Stomatology

基　　金：江苏省自然科学基金(GZK1202103)。

摘　　要：目的探讨头颈部肿瘤放疗患者,在放疗前中后期口腔微生物的动态变化及其与放射性口腔黏膜炎(radiotherapy⁃induced oral mucositis,RIOM)进展的关系。方法基于16S rRNA基因测序,分析患者口腔微生物的动态变化特征。结果厚壁菌门、变形菌门、拟杆菌门等始终构成了口腔主要优势菌。微生物的绝对丰度没有明显改变,但相对丰富度和均匀度发生了显著变化。CCA/RDA及Kruskal Wallis分析示:放疗后期链球菌属及乳杆菌属的构成比例显著上升且差异具有统计学意义。结论口腔菌群失调与RIOM有关,菌群失调会加重RIOM的严重程度,链球菌属及乳杆菌属可能与RIOM的病情进展密切相关,有待进一步动物实验验证。Objective To investigate dynamic shifts in the oral microbiota and their association with the progression and aggravation of radiotherapy⁃induced oral mucositis(RIOM)in patients with head and neck malignancies undergoing radiotherapy.Methods Based on 16S rRNA gene sequencing,dynamic changes of oral microbiota in patients was analyzed.Results Firmicutes,Bacteroidetes,and Actinobacteria consistently constituted the predominant bacteria in the oral cavity.While there were no significant changes in the abso⁃lute abundance of microbiota,there were notable alterations in their relative richness and evenness.Analyses including CCA/RDA and Kruskal⁃Wallis indicated a significant increase in the proportions of Streptococcus and Lactobacillus genera in the later stages post⁃radio⁃therapy,showing statistically meaningful differences.Conclusion RIOM is associated with dysbiosis of oral microbiota.Microbial dys⁃biosis may aggravate the severity of RIOM.Streptococcus and Lactobacillus could be closely related to the progression of RIOM,pending further validation through animal experiments.

关 键 词：口腔微生物 头颈部肿瘤 口腔黏膜炎 放射治疗 

分 类 号：R780.1[医药卫生—口腔医学] R739.8[医药卫生—临床医学]