LncRNA MEG3过表达对人子宫内膜癌Ishikawa细胞增殖和EMT的影响  

Effects of LncRNA MEG3 overexpression on proliferation and EMT of Ishikawa cells in human endometrial carcinoma

作　　者：周丽娜 杨彩虹 刘瑞 宋琳琳 张雪玉 ZHOU Linan;YANG Caihong;LIU Rui;SONG Linlin;ZHANG Xueyu(Cardiovascular and Cerebrovascular Disease Hospital,General Hospital of Ningxia Medical University,Yinchuan 750004,China)

机构地区：[1]宁夏医科大学总医院心脑血管病医院,宁夏银川750000

出　　处：《宁夏医学杂志》2024年第8期672-675,F0003,共5页Ningxia Medical Journal

基　　金：宁夏医科大学校级课题(XM2020195)。

摘　　要：目的研究LncRNA MEG3过表达对人子宫内膜癌Ishikawa细胞增殖和上皮-间充质转化(EMT)的影响。方法将Ishikawa细胞分为Control组(无转染)、Len-nc组(转染慢病毒空载体)和Len-MEG3组(转染LncRNA MEG3过表达慢病毒),采用qRT-PCR检测各组Ishikawa细胞中LncRNA MEG3、E-cadherin、N-cadherin、Vimentin和ZEB1的表达水平,通过细胞划痕实验测定各组的迁移能力。细胞的增殖能力通过克隆实验和CCK8进行测定。采用流式细胞术测定细胞凋亡情况。Western blot检测EMT相关蛋白E-cadherin、N-cadherin、Vimentin、ZEB1的表达量变化。结果Len-MEG3组与Control组、Len-nc组比较,Len-MEG3组中LncRNA MEG3的表达水平增加(P<0.05),引起细胞的迁移和增殖能力下降,提高细胞凋亡能力,同时上调了E-cadherin mRNA和蛋白的表达以及下调N-cadherin、Vimentin和ZEB1 mRNA和蛋白的表达。结论人子宫内膜癌Ishikawa细胞中LncRNA MEG3过表达可以抑制细胞的增殖、迁移和EMT,并促进细胞凋亡。Objective To investigate the effect of LncRNA MEG3 overexpression on the proliferation of Ishikawa cell in endometrial cancer and its role in the process of epithelial mesenchymal transition(EMT).Methods Ishikawa cells were divided into Control group(blank control),Len-nc group(transfection of lentiviral empty vector)and Len-MEG3 group(transfection of LncRNA MEG3 overexpression lentivirus).The expression levels of LncRNA MEG3,E-cadherin,N-cadherin,Vimentin and ZEB1 in each group of Ishikawa cells were detected by qRT-PCR,and scratch assay was used to detect the migration ability of cells in each group.CCK8 and clonal formation assay were used to detect the proliferation ability of cells in each group.Flow cytometry was used to detect the apoptosis of cells in each group.The Western blot method was performed to detect the expression levels of EMT-related proteins including E-cadherin,N-cad-herin,Vimentin and ZEB1.Result Compared with the Control group and the Len-nc group,LncRNA MEG3 expression levels were significantIy increased in the Len-MEG3 group(P<0.05),the migration and proliferation ability of cells were decreased,and the apoptosis ability of cell was increased.While the expression of E-cadherin mRNA and protein was up-regulated and the expression of N-cadherin,Vimentin and ZEB1 mRNA and protein was down-regulated.Conclusion LncRNA MEG3 overexpression inhibits the proliferation,migration ability and EMT process of Ishikawa cells in endometria cancer,while promoting apoptosis.

关 键 词：子宫内膜癌 LncRNA 上皮间质转化 

分 类 号：R737[医药卫生—肿瘤]