基于β-环糊精基金属有机框架构建4-萜烯醇包封体系  

Construction of Terpinen-4-ol Encapsulation System Based onβ-Cyclodextrin-based Metal-organic Framework

作　　者：宋佳璇 韩晨瑞 王雨贝 曹建康[1] SONG Jiaxuan;HAN Chenrui;WANG Yubei;CAO Jiankang(College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083,China)

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083

出　　处：《食品工业科技》2024年第18期88-94,共7页Science and Technology of Food Industry

基　　金：国家重点研发计划项目(2022YFF1100904);中国农业大学省校合作项目(201904710611628)。

摘　　要：为了改善4-萜烯醇的疏水性和高挥发性,延长其作用时间,本研究通过改良蒸汽扩散法制备了β-环糊精基金属有机框架(β-cyclodextrin-based metal-organic framework,β-CD-MOF),采用溶剂孵化法制备了4-萜烯醇/β-CD-MOF包合物,并以β-CD为对照探究了二者的最佳包封比例。进一步分析了4-萜烯醇/β-CD-MOF包合物的形貌结构特性、光谱吸收特性和热重表征,利用体外抑菌实验测定了该包合物对三种食源性病原菌的抑制作用。结果表明,当4-萜烯醇与β-CD-MOF的质量比为1:1时,载药量和包封率最高,且优于相同制备条件下以β-CD为载体时的效果。扫描电镜观察表明,排列不规则的β-CD分子在K^(+)的桥连作用下相互堆叠形成排列整齐的长方体β-CD-MOF。傅里叶红外光谱和X射线衍射分析表明,4-萜烯醇成功封装在β-CD-MOF中且二者之间存在氢键相互作用。热重分析表明,利用β-CD-MOF包封可以延缓4-萜烯醇的挥发速率,将4-萜烯醇应用的温度范围从30~160℃扩大到30~350℃。体外抑菌实验表明,4-萜烯醇/β-CD-MOF包合物对大肠杆菌、金黄色葡萄球菌和单增李斯特菌三种冷链微生物均具有长效抑菌能力。综上所述,将4-萜烯醇封装在β-CD-MOF中可以实现有效保存,本研究为绿色安全的新型冷链抑菌材料的开发提供了理论依据。In order to improve the hydrophobicity and volatility of terpinen-4-ol and extend its duration of action,β-cyclodextrin-based metal-organic framework(β-CD-MOF)was first prepared by the modified vapor diffusion method,and then terpinen-4-ol was encapsulated with theβ-CD-MOF to prepare a terpinen-4-ol/β-CD-MOF inclusion complex by the solvent incubation method.The mass ratio of the preparedβ-CD-MOF orβ-CD used as the control to terpinen-4-ol was optimized for encapsulation.The morphological characteristics,spectral absorption properties and thermogravimetric characterisation of the terpinen-4-ol/β-CD-MOF complex were analyzed.Through in vitro bacteriostatic assays,the inhibitory effects of the complex on three food-borne pathogens were determined.The results showed that the highest drug loading and encapsulation efficiency were achieved when the mass ratio of terpinen-4-ol toβ-CD-MOF was 1:1,superior to the same preparation conditions usingβ-CD as a carrier.Scanning electron microscope(SEM)observations indicated that irregularly arrangedβ-CD molecules stacked together under the bridging action of K^(+),forming an orderly arranged rectangularβ-CD-MOF.Fourier-transform infrared spectroscopy(FTIR)and X-ray diffraction(XRD)analysis demonstrated terpinen-4-ol was successfully encapsulated inβ-CD-MOF with hydrogen bond interactions between them.Thermogravimetric analysis(TGA)proved that the volatilization rate of terpinen-4-ol could be retarded when encapsulated byβ-CD-MOF,extending its application temperature range from 30~160℃to 30~350℃.In vitro antibacterial experiments showed that the terpinen-4-ol/β-CD-MOF complex exhibited long-lasting antibacterial abilities against three common cold-chain microorganisms,including Escherichia coli,Staphylococcus aureus and Listeria monocytogenes.In conclusion,encapsulating terpinen-4-ol inβ-CD-MOF allows for effective preservation,providing a theoretical basis for the development of novel,green and safe antimicrobial materials for cold-chain applications.

关 键 词：β-CD-MOF 4-萜烯醇 包封 表征 稳定性 抑菌活性 

分 类 号：TS255.3[轻工技术与工程—农产品加工及贮藏工程]