B细胞易位基因1对B细胞淋巴瘤增殖的影响  

Effect of B cell translocation gene 1 on proliferation of lymphoma B cells

作　　者：赵瑾[1] 温晓莲[1] 关涛[1] 郑美婧[1] 马莉[1] 苏丽萍[1] Zhao Jin;Wen Xiaolian;Guan Tao;Zheng Meijing;Ma Li;Su Liping(Department of Hematology,Shanxi Cancer Hospital,Chinese Academy of Medical Sciences Cancer Hospital Shanxi Hospital,Shanxi Medical University Affiliated Cancer Hospital,Taiyuan 030013,China)

机构地区：[1]山西省肿瘤医院、中国医学科学院肿瘤医院山西医院、山西医科大学附属肿瘤医院血液内科,太原030013

出　　处：《中华实验外科杂志》2024年第8期1721-1724,共4页Chinese Journal of Experimental Surgery

基　　金：山西省基础研究计划项目(202203021222388)。

摘　　要：目的探讨B细胞易位基因1(BTG1)在SU-DHL-2细胞中的表达及其通过自噬对细胞增殖和凋亡的影响。方法实时荧光定量反转录聚合酶链反应(RT-qPCR)和蛋白印迹法分别检测人弥漫大B细胞淋巴瘤细胞系U2932、OCI-LY-10、SU-DHL-2以及B淋巴母细胞IM-9中BTG1 mRNA和蛋白表达量。SU-DHL-2细胞按照随机数字表法分为对照组、空载体组、BTG1过表达组、3-MA组和BTG1过表达+3-MA组,细胞计数试剂盒(CCK-8)检测细胞增殖活性,流式细胞仪检测细胞凋亡率,蛋白印迹法检测微管相关蛋白轻链3Ⅱ(LC3Ⅱ)、酵母Atg6同系物(Beclin1)和自噬相关蛋白5(Atg5)表达量。多组间比较采用单因素方差分析(One way-ANOVA),组间两两比较采用LSD-t检验。结果U2932、OCI-LY-10、SU-DHL-2细胞中BTG1 mRNA表达量(0.85±0.06、0.54±0.06、0.53±0.05)均低于IM-9细胞(1.22±0.16,t=3.750、6.893、7.129,P<0.05),蛋白表达量(0.70±0.08、0.54±0.06、0.24±0.04)均低于IM-9细胞(0.87±0.06,t=2.944、6.736、15.132,P<0.05)。BTG1过表达组细胞增殖活性[(74.80±1.24)%]低于对照组(100.00%,t=35.200,P<0.05)和空载体组[(98.35±1.02)%,t=25.404,P<0.05];BTG1过表达组细胞凋亡率[(14.74±0.93)%]高于对照组[(6.28±0.31)%,t=14.948,P<0.05]和空载体组[(8.53±0.55)%,t=9.955,P<0.05];BTG1过表达组BTG1 mRNA表达量(2.33±0.08)高于对照组(1.03±0.06,t=22.517,P<0.05)和空载体组(1.01±0.07,t=21.508,P<0.05),蛋白表达量(0.88±0.07)高于对照组(0.56±0.07,t=5.599,P<0.05)和空载体组(0.61±0.06,t=5.072,P<0.05)。BTG1过表达+3-MA组细胞增殖活性[(84.21±3.26)%]高于BTG1过表达组[(73.42±3.48)%,t=3.919,P<0.05],细胞凋亡率[(18.40±2.26)%]低于BTG1过表达组[(26.79±2.80)%,t=4.039,P<0.05],LC3Ⅱ、Beclin1和Atg5蛋白表达量(0.56±0.05、0.72±0.07、0.49±0.05)低于BTG1过表达组(0.71±0.06、0.89±0.05、0.68±0.05,t=3.326、3.423、4.654,P<0.05)。结论BTG1过表达可诱导自噬发挥抗弥漫大B细胞淋巴瘤作用。ObjectiveTo investigate the expression of B-cell translocation gene 1(BTG1)in diffuse large B-cell lymphoma and its effect on the proliferation and apoptosis of SU-DHL-2 cells by inducing autophagy.MethodsThe expression levels of BTG1 mRNA and protein in diffuse large B-cell lymphoma cell lines U2932,OCI-LY-10,SU-DHL-2 and B lymphoblast-9 were detected by real-time quantitative reverse transcription polymerase chain reaction(RT-qPCR)and Western blotting,respectively.SU-DHL-2 cells were randomly divided into control group,empty carrier group,BTG1 overexpression group,3-MA group and BTG1 overexpression+3-MA group.Cell proliferation activity was detected by cell counting kit-8(CCK-8)method,cell apoptosis rate was detected by flow cytometry,and protein expression levels of LC3Ⅱ,Atg5 and Beclin1 were detected by Western blotting.One way ANOVA was used for multi-group comparison,and LSD-t test was used for pairwise comparison between groups.ResultsThe mRNA expression of BTG1 in U2932,OCI-LY-10 and SU-DHL-2 cells(0.85±0.06,0.54±0.06,0.53±0.05)was lower than that in IM-9 cells(1.22±0.16,t=3.750,6.893,7.129,and P<0.05).Protein expression levels in U2932,OCI-LY-10 and SU-DHL-2 cells(0.70±0.08,0.54±0.06,0.24±0.04)were all lower than those in IM-9 cells(0.87±0.06,t=2.944,6.736,15.132,all P<0.05).The cell proliferation activity of BTG1 overexpression group[(74.80±1.24)%]was significantly lower than that of control group(100.00%,t=35.200,P<0.05),and empty carrier group[(98.35±1.02)%,t=25.404,P<0.05].The apoptosis rate of BTG1 overexpression group[(14.74±0.93)%]was significantly higher than that of control group[(6.28±0.31)%,t=14.948,P<0.05],and empty carrier group[(8.53±0.55)%,t=9.955,P<0.05].The mRNA expression of BTG1 in overexpression group(2.33±0.08)was significantly higher than that in control group(1.03±0.06,t=22.517,P<0.05),and empty carrier group(1.01±0.07,t=21.508,P<0.05).Protein expression level of BTG1 in overexpression group(0.88±0.07)was significantly higher than that of control group(0.56±0.07

关 键 词：淋巴瘤 自噬 增殖 凋亡 

分 类 号：R733.1[医药卫生—肿瘤]